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Literature summary for 3.4.21.37 extracted from
- A kinetic study of the hydrolysis of the N-tosylalanine ester of 3-hydroxy-5-phenylpyrrole and related compounds by human leukocyte elastase (1995), Arch. Biochem. Biophys., 323, 108-114.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the tosylalanine ester of 3-hydroxy-5-phenylpyrrole is localized on reagent strips and used diagnostically to test urine for the presence of human leukocyte elastase as an indication of urinary tract infection | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 9.0 | Homo sapiens |  |
| 0.016 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0 or pH 9.0 | Homo sapiens | |
| 0.017 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0 | Homo sapiens | |
| 0.022 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0 | Homo sapiens | |
| 0.026 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0 | Homo sapiens | |
| 0.028 | - |
tosyl-Ala-4-nitrophenol | pH 8.0 | Homo sapiens | |
| 0.035 | - |
tosyl-Ala-4-nitrophenol | pH 7.0 | Homo sapiens | |
| 0.046 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 9.0 | Homo sapiens | |
| 0.054 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0, in presence of 0.35 mM decanol | Homo sapiens | |
| 0.056 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 7.0 | Homo sapiens | |
| 0.065 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 8.0 | Homo sapiens | |
| 0.067 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0, in presence of 0.35 mM decanol | Homo sapiens | |
| 0.08 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 9.0, in presence of 0.35 mM decanol | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole + H2O | - |
Homo sapiens | ? | - |
? | |
| benzyloxycarbonyl-Ala-4-nitrophenol + H2O | - |
Homo sapiens | benzyloxycarbonyl-Ala + 4-nitrophenol | - |
? | |
| tosyl-Ala-3-hydroxy-5-phenylpyrrole + H2O | - |
Homo sapiens | ? | - |
? | |
| tosyl-Ala-4-nitrophenol + H2O | - |
Homo sapiens | tosyl-Ala + 4-nitrophenol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0 | Homo sapiens | |
| 18 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 9.0 | Homo sapiens | |
| 20 | - |
benzyloxycarbonyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0 | Homo sapiens | |
| 28 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 7.0 | Homo sapiens | |
| 36 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 9.0 | Homo sapiens | |
| 46 | - |
benzyloxycarbonyl-Ala-4-nitrophenol | pH 8.0 | Homo sapiens | |
| 96 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 9.0 | Homo sapiens | |
| 110 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0 | Homo sapiens | |
| 120 | - |
tosyl-Ala-4-nitrophenol | pH 7.0 and pH 8.0 | Homo sapiens | |
| 140 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0 | Homo sapiens | |
| 4200 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 9.0, in presence of 0.35 mM decanol | Homo sapiens | |
| 4500 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 7.0, in presence of 0.35 mM decanol | Homo sapiens | |
| 4800 | - |
tosyl-Ala-3-hydroxy-5-phenylpyrrole | pH 8.0, in presence of 0.35 mM decanol | Homo sapiens | |
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