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Literature summary for 3.4.21.37 extracted from
- Structural and functional characterization of elastases from horse neutrophils (1994), Biochem. J., 300, 401-406.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ala15-reactive site variant of aprotinin | good inhibitor for proteinase 2B and weak inhibitor of proteinase 2A | Equus caballus | |
| alpha-aminobutyric acid15-reactive site variant of aprotinin | good inhibitor for proteinase 2B and weak inhibitor of proteinase 2A | Equus caballus | |
| alpha-aminovaleric acid15-reactive site variant of aprotinin | good inhibitor for proteinase 2B and weak inhibitor of proteinase 2A | Equus caballus | |
| Aprotinin | no inhibition of proteinase 2A and 2B | Equus caballus |  |
| Aprotinin | - |
Homo sapiens | |
| Val15-reactive site variant of aprotinin | good inhibitor for proteinase 2B and weak inhibitor of proteinase 2A | Equus caballus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester | pH 7.5, 25°C, proteinase B | Equus caballus | |
| 0.04 | - |
tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 0.12 | - |
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 0.2 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C | Equus caballus | |
| 0.27 | - |
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 0.3 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 0.6 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 1.1 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 2.4 | - |
methoxysuccinyl-Ala-Ala-Pro-Met-4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 2.8 | - |
N-succinyl-Ala-Ala-Val 4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 3 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 3.2 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 3.3 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 27.8 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Equus caballus | - |
proteinase 2A and 2B | - |
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methoxysuccinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
| methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O | - |
Homo sapiens | ? | - |
? | |
| methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O | - |
Equus caballus | ? | - |
? | |
| N-succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Homo sapiens | N-succinyl-Ala-Ala-Ala + 4-nitroaniline | - |
? | |
| N-succinyl-Ala-Ala-Ala 4-nitroanilide + H2O | - |
Equus caballus | N-succinyl-Ala-Ala-Ala + 4-nitroaniline | - |
? | |
| N-succinyl-Ala-Ala-Val 4-nitroanilide + H2O | - |
Homo sapiens | N-succinyl-Ala-Ala-Val + 4-nitroaniline | - |
? | |
| N-succinyl-Ala-Ala-Val 4-nitroanilide + H2O | - |
Equus caballus | N-succinyl-Ala-Ala-Val + 4-nitroaniline | - |
? | |
| oxidized B chain of insulin + H2O | initial cleavage sites for proteinase 2A and 2B are the Ala14-Leu15 and Val18-Cys19 bonds. Cleavage of the Val12-Glu13 bond is a secondary event resulting from the further cleavage of the primary peptide Phe1-Ala14 | Homo sapiens | ? | - |
? | |
| tert-butyloxycarbonyl-Ala-4-nitrophenyl ester + H2O | - |
Homo sapiens | tert-butyloxycarbonyl-Ala + 4-nitrophenol | - |
? | |
| tert-butyloxycarbonyl-Ala-4-nitrophenyl ester + H2O | - |
Equus caballus | tert-butyloxycarbonyl-Ala + 4-nitrophenol | - |
? | |
| tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester + H2O | - |
Equus caballus | tert-butyloxycarbonyl-L-Val + 4-nitrophenol | - |
? | |
| tert-butyloxycarbonyl-Val-4-nitrophenyl ester + H2O | - |
Homo sapiens | tert-butyloxycarbonyl-Val + 4-nitrophenol | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 0.72 | - |
methoxysuccinyl-Ala-Ala-Pro-Met-4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 1.6 | - |
N-succinyl-Ala-Ala-Val 4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 2.5 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 2.5 | - |
tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 3.1 | - |
N-succinyl-Ala-Ala-Val 4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Homo sapiens | |
| 3.4 | - |
N-succinyl-Ala-Ala-Ala 4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 4.5 | - |
tert-butyloxycarbonyl-L-Val-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 6.2 | - |
N-succinyl-Ala-Ala-Val 4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 9.5 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
| 10.8 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C | Homo sapiens | |
| 12 | - |
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 17.9 | - |
methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide | pH 7.5, 25°C, proteinase 2B | Equus caballus | |
| 26.1 | - |
tert-butyloxycarbonyl-Ala-4-nitrophenyl ester | pH 7.5, 25°C, proteinase 2A | Equus caballus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000002 | - |
alpha-aminobutyric acid15-reactive site variant of aprotinin | pH 7.4, 25°C | Homo sapiens | |
| 0.0000002 | - |
alpha-aminovaleric acid15-reactive site variant of aprotinin | pH 7.4, 25°C | Homo sapiens | |
| 0.0000003 | - |
Val15-aminobutyric acid15-reactive site variant of aprotinin | pH 7.4, 25°C | Homo sapiens | |
| 0.0000004 | - |
alpha-aminobutyric acid15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2B | Equus caballus | |
| 0.0000011 | - |
alpha-aminovaleric acid15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2B | Homo sapiens | |
| 0.0000018 | - |
Ala15-reactive site variant of aprotinin | pH 7.4, 25°C | Homo sapiens | |
| 0.0000018 | - |
Ala15-reactive site variant of aprotinin | pH 7.4, 25°C | Equus caballus | |
| 0.0000018 | - |
Ala15-reactive site variant of aprotinin | proteinase 2B | Equus caballus | |
| 0.000004 | - |
Val15-reactive site variant of aprotinin | - |
Equus caballus | |
| 0.000004 | - |
Val15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2B | Homo sapiens | |
| 0.00012 | - |
Val15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2A | Homo sapiens | |
| 0.00022 | - |
alpha-aminovaleric acid15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2A | Homo sapiens | |
| 0.00037 | - |
alpha-aminobutyric acid15-reactive site variant of aprotinin | pH 7.4, 25°C, proteinase 2A | Homo sapiens |
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