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Literature summary for 3.4.21.35 extracted from

  • Luo, L.Y.; Shan, S.J.; Elliott, M.B.; Soosaipillai, A.; Diamandis, E.P.
    Purification and characterization of human kallikrein 11, a candidate prostate and ovarian cancer biomarker, from seminal plasma (2006), Clin. Cancer Res., 12, 742-750.
    View publication on PubMed
Search for more literature for 3.4.21.35

Inhibitors

Inhibitors Comment Organism Structure
(4-amidino-phenyl)-methane-sulfonyl fluoride 2.5 mM, complete inhibition Homo sapiens
Aprotinin up to 40% inhibition Homo sapiens
hK11-specific monoclonal antibody up to 40% inhibition Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.28
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.44
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.53
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
1.18
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 about 40% of human tissue kallikrein 11 (40000 Da) is enzymatically active, whereas the rest is inactivated by internal cleavage after Arg156 which generates two peptides of 20000 Da, connected by internal disulfide bonds Homo sapiens
40000
-
 x * 40000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBX7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
seminal plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information the enzyme cleaves synthetic peptides after arginine but not lysine residues. It does not cleave chymotrypsin Homo sapiens ?
-
 ?
Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Phe-Ser-Arg + 7-amino-4-methylcoumarin
-
 ?
Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Val-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Val-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hK11
-
 Homo sapiens
KLK11
-
 Homo sapiens
tissue kallikrein 11
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0035
-
 Gly-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.0038
-
 Gln-Ala-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.0065
-
 Phe-Ser-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.0075
-
 Val-Pro-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens
0.031
-
 Pro-Phe-Arg-7-amido-4-methylcoumarin 37°C, pH 7.5 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 hydrolysis of Pro-Phe-Arg-7-amido-4-methylcoumarin Homo sapiens