Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.35 extracted from

  • Fiedler, F.
    Pig pancreatic kallikrein A and B (1976), Methods Enzymol., 45, 289-303.
    View publication on PubMed
Search for more literature for 3.4.21.35

Inhibitors

Inhibitors Comment Organism Structure
cinnamoyl imidazole
-
 Sus scrofa
diisopropyl fluorophosphate
-
 Sus scrofa
indolacryloyl imidazole
-
 Sus scrofa
p-nitrophenyl p'-guanidinobenzoate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 N-alpha-toluenesulfonyl-L-Arg methyl ester pH 8.0, 40°C Sus scrofa
0.104
-
 N-alpha-benzoyl-L-Arg ethyl ester pH 8.0, 40°C Sus scrofa
0.125
-
 N-alpha-benzoyl-L-Arg methyl ester pH 8.0, 40°C Sus scrofa
2
-
 N-alpha-benzoyl-L-Lys methyl ester pH 8.0, 40°C Sus scrofa
2.44
-
 N-alpha-benzoyl-L-Orn methyl ester pH 8.0, 40°C Sus scrofa
12.3
-
 N-alpha-benzoyl-L-citrulline methyl ester pH 8.0, 40°C Sus scrofa
50
-
 N-alpha-acetyl-Tyr ethyl ester pH 8.0, 40°C Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000 35000 gel filtration Sus scrofa
33500
-
 light scattering Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polypeptide + H2O Sus scrofa
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification kallikrein A contains 5.6% carbohydrate by weight, kallikrein B contains 11.5% carbohydrate per weight. Carbohydrate is located on both chains of kallikrein B Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
kallikrein A and B Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-acetyl-Tyr ethyl ester + H2O
-
 Sus scrofa N-alpha-acetyl-Tyr + ethanol
-
 ?
N-alpha-benzoyl-L-Arg ethyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Arg + ethanol
-
 ?
N-alpha-benzoyl-L-Arg methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Arg + methanol
-
 ?
N-alpha-benzoyl-L-citrulline methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-citrulline + methanol
-
 ?
N-alpha-benzoyl-L-Lys methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Lys + methanol
-
 ?
N-alpha-benzoyl-L-Orn methyl ester + H2O
-
 Sus scrofa N-alpha-benzoyl-L-Orn + methanol
-
 ?
polypeptide + H2O
-
 Sus scrofa peptides
-
 ?
Tosyl-Arg methyl ester + H2O
-
 Sus scrofa Tosyl-Arg + methanol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 N-alpha-benzoyl-L-Orn methyl ester pH 8.0, 25°C Sus scrofa
2.7
-
 N-alpha-toluenesulfonyl-L-Arg methyl ester pH 8.0, 25°C Sus scrofa
11.2
-
 N-alpha-acetyl-Tyr ethyl ester pH 8.0, 25°C Sus scrofa
15.5
-
 N-alpha-benzoyl-L-Lys methyl ester pH 8.0, 25°C Sus scrofa
86.4
-
 N-alpha-benzoyl-L-citrulline methyl ester pH 8.0, 25°C Sus scrofa
123
-
 N-alpha-benzoyl-L-Arg ethyl ester pH 8.0, 25°C Sus scrofa
154
-
 N-alpha-benzoyl-L-Arg methyl ester pH 8.0, 25°C Sus scrofa