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Literature summary for 3.4.21.32 extracted from

  • Kim, S.K.; Park, P.J.; Kim, J.B.; Shahidi, F.
    Purification and characterization of a collagenolytic protease from the filefish, Novoden modestrus (2002), J. Biochem. Mol. Biol., 35, 165-171.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ 0.06 mM, 29% inhibition Novoden modestrus
Cu2+ 0.06 mM, 26% inhibition Novoden modestrus
diisopropylfluorophosphate 0.2 mM, complete inhibition Novoden modestrus
EDTA 0.2 mM, 46% inhibition. 0.5 mM, 95% inhibition Novoden modestrus
Hg2+ 0.06 mM, 19% inhibition Novoden modestrus
His 0.2 mM, 51% inhibition. 0.7 nmM, 93% inhibition Novoden modestrus
Mn2+ 0.06 mM, 14% inhibition Novoden modestrus
Ni2+ 0.06 mM, 30% inhibition Novoden modestrus
Soybean trypsin inhibitor 0.2 mM, complete inhibition Novoden modestrus
tosyl-L-Leu-chloromethylketone 0.2 mM, complete inhibition Novoden modestrus
Zn2+ 0.06 mM, 36% inhibition Novoden modestrus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 0.06 mM, activation to 119% of control Novoden modestrus
Ca2+ 0.06 mM, activation to 135% of control Novoden modestrus
K+ 0.06 mM, activation to 123% of control Novoden modestrus
Li+ 0.06 mM, activation to 111% of control Novoden modestrus
Mg2+ 0.06 mM, activation to 111% of control Novoden modestrus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
gel filtration Novoden modestrus

Organism

Organism UniProt Comment Textmining
Novoden modestrus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Novoden modestrus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10550
-
-
Novoden modestrus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Collagen + H2O
-
Novoden modestrus Hydrolyzed collagen
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, SDS-PAGE Novoden modestrus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
hydrolysis of collagen Novoden modestrus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 60 45°C: about 40% of maximal activity, 60°C: about 45% of maximal activity, hydrolysis of collagen Novoden modestrus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8 hydrolysis of collagen Novoden modestrus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 pH 6.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of collagen Novoden modestrus