Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 0.06 mM, 29% inhibition | Novoden modestrus | |
Cu2+ | 0.06 mM, 26% inhibition | Novoden modestrus | |
diisopropylfluorophosphate | 0.2 mM, complete inhibition | Novoden modestrus | |
EDTA | 0.2 mM, 46% inhibition. 0.5 mM, 95% inhibition | Novoden modestrus | |
Hg2+ | 0.06 mM, 19% inhibition | Novoden modestrus | |
His | 0.2 mM, 51% inhibition. 0.7 nmM, 93% inhibition | Novoden modestrus | |
Mn2+ | 0.06 mM, 14% inhibition | Novoden modestrus | |
Ni2+ | 0.06 mM, 30% inhibition | Novoden modestrus | |
Soybean trypsin inhibitor | 0.2 mM, complete inhibition | Novoden modestrus | |
tosyl-L-Leu-chloromethylketone | 0.2 mM, complete inhibition | Novoden modestrus | |
Zn2+ | 0.06 mM, 36% inhibition | Novoden modestrus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 0.06 mM, activation to 119% of control | Novoden modestrus | |
Ca2+ | 0.06 mM, activation to 135% of control | Novoden modestrus | |
K+ | 0.06 mM, activation to 123% of control | Novoden modestrus | |
Li+ | 0.06 mM, activation to 111% of control | Novoden modestrus | |
Mg2+ | 0.06 mM, activation to 111% of control | Novoden modestrus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
gel filtration | Novoden modestrus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Novoden modestrus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Novoden modestrus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10550 | - |
- |
Novoden modestrus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | - |
Novoden modestrus | Hydrolyzed collagen | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, SDS-PAGE | Novoden modestrus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
hydrolysis of collagen | Novoden modestrus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | 45°C: about 40% of maximal activity, 60°C: about 45% of maximal activity, hydrolysis of collagen | Novoden modestrus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | hydrolysis of collagen | Novoden modestrus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | pH 6.5: about 60% of maximal activity, pH 8.5: about 50% of maximal activity, hydrolysis of collagen | Novoden modestrus |