Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Factor VIIIa | - |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | an FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa cleaves a FIXa-specific chromogenic substrate with normal catalytic efficiency, however, the mutant exhibits about 5fold enhanced reactivity with antothrombin specifically in the absence of the cofactor, heparin. The FIXa mutant activates factor X with about 4fold decreased kcat and about 2fold decreased Km, although the mutant interacts normally with factor VIIIa | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000038 | - |
Factor X | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens | |
0.000071 | - |
Factor X | wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens | |
1.2 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens | |
2.3 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | Homo sapiens | - |
Factor Xa + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Factor X + H2O | - |
Homo sapiens | Factor Xa + ? | - |
? | |
L-Leu-Gly-L-Arg-4-nitroanilide + H2O | CBS 31.39 | Homo sapiens | L-Leu-Gly-L-Arg + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor IXa | - |
Homo sapiens |
FIXa | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
Factor X | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens | |
3.5 | - |
Factor X | wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens | |
17.5 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens | |
21.4 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | wild type enzyme, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens | |
14.6 | - |
L-Leu-Gly-L-Arg-4-nitroanilide | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in 0.02 M Tris-HCl (pH 7.5) buffer containing 0.1 M NaCl, 0.1 mg/ml bovine serum albumin, 33% ethylene glycol, and 5 mM Ca2+, at 22°C | Homo sapiens | |
25000 | - |
Factor X | FIXa/FXa chimera in which the 39-loop of the protease is replaced with the corresponding loop of FXa, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens | |
49000 | - |
Factor X | wild type enzyme, in TBS containing 0.1 mg/ml bovine serum albumin, 0.1% polyethylene glycol (PEG) 8000, and 5 mM Ca2+, at 22°C, pH not specified in the publication | Homo sapiens |