Activating Compound | Comment | Organism | Structure |
---|---|---|---|
anionic phospholipid | highly activating | Homo sapiens | |
vitamin K | dependent on | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K98A | site-directed mutagenesis, | Homo sapiens |
additional information | the modulatory effect of heparin can be completely abrogated by mutating a single amino acid residue in the 99-loop region of the extended fIXa active site cleft outside of the heparin binding exosite, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin | in vivo and in vitro inhibition, the interaction is improved by heparin binding via the formation of bridged complexes where heparin acts as a template | Homo sapiens | |
BPTI | Kunitz-type inhibitor, competitive, reversible inhibition, low molecular weight heparin binding to fIXa enhances reactivity of fIXa with the Kunitz-type inhibitor BPTI | Homo sapiens | |
enoxaparin | competitive, reversible inhibition | Homo sapiens | |
additional information | oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region, overview | Homo sapiens | |
PN2-KPI | Kunitz-type inhibitor, competitive, reversible inhibition | Homo sapiens | |
TFPI-K1 | Kunitz-type inhibitor, competitive, reversible inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | hughly activating | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
factor X + H2O | Homo sapiens | factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation | activated factor X + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00740 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | activation by purified factor X activator from the venom of Russells Vipe cleaving fIX after Arg180 to generate active enzyme fIXaalpha | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from HEK-293 cells by anion exchange and heparin affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | from plasma | Homo sapiens | - |
plasma | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CH3SO2-D-Leu-Gly-Arg-4-nitroanilide + H2O | commercial chromogenic substrate CBS 31.39 | Homo sapiens | ? | - |
? | |
factor X + H2O | factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation | Homo sapiens | activated factor X + ? | - |
? | |
factor X + H2O | oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region | Homo sapiens | activated factor X + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
factor IXa | - |
Homo sapiens |
FIXa | - |
Homo sapiens |
More | the enzyme is a member of the serine protease family and shares a high degree of homology with trypsin | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Homo sapiens |