Any feedback?
Literature summary for 3.4.21.22 extracted from
- The heparin-binding exosite is critical to allosteric activation of factor IXa in the intrinsic tenase complex: the role of arginine 165 and factor X (2007), Biochemistry, 46, 7886-7895.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Factor VIIIa | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex | Homo sapiens | |
| heparin | activates by mediating cofactor binding, heparin binds to an exosite on the factor IXa protease domain, the factor IXa heparin-binding exosite participates in both cofactor binding and protease activation, and cofactor affinity is linked to active site conformation and factor X interaction during enzyme assembly, overview | Homo sapiens |  |
| Phospholipids | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable expression of wild-type and mutant enzymes in HEK-293 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K126A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
| K132A | site-directed mutagenesis, surface residue mutation, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
| N129A | site-directed mutagenesis, surface residue mutation, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
| N178A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
| R165A | site-directed mutagenesis, mutation in the heparin-binding exosite, the mutant shows reduced activity and altered tenase complex formation kinetics compared to the wild-type enzyme | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| antithrombin III | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic determination of the affinity for factor IXa-factor VIIIa complex formation, effect of ethylene glycol at 30% on reaction kinetics, overview | Homo sapiens | |
| 0.000001 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 0.0000052 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 0.0000073 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 0.0000085 | - |
Factor X | pH 7.4, 22°C, recombinant mutants N129A and K132A, each in the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 0.0000096 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 0.000048 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 0.000055 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 0.000061 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N129A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 0.000067 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 0.00008 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 0.000089 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Homo sapiens | |
| Na+ | activates | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor X + H2O | Homo sapiens | the enzyme is part of the coagulation cascade, factor X activation by the intrinsic tenase complex, factor IXa-factor VIIIa, is the rate-limiting step for thrombin generation | activated factor X + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | purified factor IX is activated with human factor XIa | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | from plasma | Homo sapiens | - |
| plasma | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
relative coagulation and amidolytic activities of recombinant wild-type and mutant enzymes, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| factor X + H2O | the enzyme is part of the coagulation cascade, factor X activation by the intrinsic tenase complex, factor IXa-factor VIIIa, is the rate-limiting step for thrombin generation | Homo sapiens | activated factor X + ? | - |
? | |
| factor X + H2O | factor IXa acts as part of phosphocholine/phosphoserine vesicles interacting with factor VIIIa in the intrinsic tenase complex, the factor IXa heparin-binding exosite participates in both cofactor binding and protease activation, and cofactor affinity is linked to active site conformation and factor X interaction during enzyme assembly, overview | Homo sapiens | activated factor X + ? | - |
? | |
| Pefachrome IXa + H2O | commercial chromogenic substrate | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| factor IXa | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 5.08 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 5.2 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 5.47 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 5.51 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 5.6 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N129A the intrinsic tenase complex, in absence of ethylene glycol | Homo sapiens | |
| 600 | - |
Factor X | pH 7.4, 22°C, recombinant mutant N178A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 1000 | - |
Factor X | pH 7.4, 22°C, recombinant mutant R165A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 1340 | - |
Factor X | pH 7.4, 22°C, recombinant wild-type enzyme and mutant N129A, each in the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 1780 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K132A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens | |
| 1999 | - |
Factor X | pH 7.4, 22°C, recombinant mutant K126A the intrinsic tenase complex, in presence of 30% ethylene glycol | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
