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Literature summary for 3.4.21.22 extracted from

  • Schmidt, A.E.; Stewart, J.E.; Mathur, A.; Krishnaswamy, S.; Bajaj, S.P.
    Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa binding (2005), J. Mol. Biol., 350, 78-91.
    View publication on PubMed
Search for more literature for 3.4.21.22

Activating Compound

Activating Compound Comment Organism Structure
factor FVIIa in complex with tissue factor and Ca2+ Homo sapiens
factor FVIIIa
-
 Homo sapiens
factor FXIa in presence of 5 mM CaCl2 Homo sapiens
Phospholipid provided by platelet surface Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
FIXagamma225P mutant enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5 mM Homo sapiens
Na+ 200 mM in the absence of Ca2+, 185 mM in the presence of 5 mM Ca2+ Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Na+ Homo sapiens
1.9
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa Homo sapiens
2.1
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P Homo sapiens
3.1
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Na+ Homo sapiens
4.5
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 5 mM Ca2+ and 185 mM Na+ Homo sapiens
7.3
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 5 mM Ca2+ and 185 mM Na+ Homo sapiens
7.9
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Ca2+ Homo sapiens
8.7
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Ca2+ Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00740
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O
-
 Homo sapiens 4-methylsulfonyl-D-Leu-Gly-Arg + p-nitroaniline
-
 ?
factor X + H2O
-
 Homo sapiens factor Xa
-
 ?
p-aminobenzamidine + H2O
-
 Homo sapiens p-aminobenzoic acid + NH3
-
 ?

Synonyms

Synonyms Comment Organism
factor IXa
-
 Homo sapiens
FIXa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P Homo sapiens
0.058
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Na+ Homo sapiens
0.42
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa Homo sapiens
0.63
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Na+ Homo sapiens
0.78
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of Ca2+ and 185 mM Na+ Homo sapiens
0.89
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide mutant enzyme FIXagamma225P, in presence of 200 mM Ca2+ Homo sapiens
3.15
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of 200 mM Ca2+ Homo sapiens
3.38
-
 4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide wild type enzyme FIXa, in presence of Ca2+ and 185 mM Na+ Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
 Na+ wild type enzyme FIXa, in the presence of 200 mM Na+ Homo sapiens
0.099
-
 Na+ wild type enzyme FIXa Homo sapiens
0.119
-
 Na+ wild type enzyme FIXa, in the presence of 185 mM Na+ and 5 mM Ca2+ Homo sapiens
0.166
-
 Na+ wild type enzyme FIXa, in the presence of 5 mM Ca2+ Homo sapiens
0.179
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 185 mM Na+ and 5 mM Ca2+ Homo sapiens
0.184
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 5 mM Ca2+ Homo sapiens
0.813
-
 Na+ mutant enzyme FIXagamma225P, in the presence of 200 mM Na+ Homo sapiens