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Literature summary for 3.4.21.22 extracted from

  • Yang, X.; Chang, Y.J.; Lin, S.W.; Walsh, P.N.
    Identification of residues Asn89, Ile90, and Val107 of the factor IXa second epidermal growth factor domain that are essential for the assembly of the factor X-activating complex on activated platelets (2004), J. Biol. Chem., 279, 46400-46405.
    View publication on PubMed
Search for more literature for 3.4.21.22

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
rFIXa104A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa105A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa107A recombinant FIXa alanine point mutation in loop 2 of the EGF2 domain Homo sapiens
rFIXa89A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa90A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa91A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens
rFIXa94A recombinant FIXa alanine point mutation in loop 1 of the EGF2 domain Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000068
-
 Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000089
-
 Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000181
-
 Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.00002
-
 Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000224
-
 Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000251
-
 Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000295
-
 Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.000034
-
 Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000396
-
 Factor X plasma-derived FIXa, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000428
-
 Factor X plasma-derived FIXa, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000629
-
 Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.000064
-
 Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000787
-
 Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0000869
-
 Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and extruded phosphatidylcholine-phosphatidylserine phospholipid vesicles supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
0.0001542
-
 Factor X mutant enzyme rFIXa89A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor X + H2O Homo sapiens in the presence of FVIIIa (5 units/ml) at 37 °C factor Xa
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P00740
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography on Q-Sepharose Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
factor X + H2O
-
 Homo sapiens factor Xa
-
 ?
factor X + H2O in the presence of FVIIIa (5 units/ml) at 37 °C Homo sapiens factor Xa
-
 ?
N-alpha-benzyloxycarbonyl-D-arginylglycyl-L-arginine-para-nitroanilide + H2O
-
 Homo sapiens N-alpha-benzyloxycarbonyl-D-arginylglycyl-L-arginine + para-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
factor IXa
-
 Homo sapiens
FIXa
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 Factor X mutant enzyme rFIXa104A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
2.9
-
 Factor X mutant enzyme rFIXa94A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
3.3
-
 Factor X mutant enzyme rFIXa107A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
3.4
-
 Factor X plasma-derived FIXa, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
3.8
-
 Factor X mutant enzyme rFIXa105A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
4.1
-
 Factor X mutant enzyme rFIXa90A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
5.2
-
 Factor X mutant enzyme rFIXa91A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens
16.6
-
 Factor X mutant enzyme rFIXa89A, 5 units/ml FVIIIa and SFLLRN-activated platelets supplemented with bovine serum albumine (2 mg/ml) and 5 mM CaCl2, at 37 °C Homo sapiens