Application | Comment | Organism |
---|---|---|
biotechnology | to overcome the defect for high-level expression of the functional recombinant coagulation FVII in Sf9 cell this method involves simultaneous expression of both human c-carboxylase and human FVII genes in the host leading to a high-level expression of functional recombinant factor VII | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Spodoptera frugiperda Sf9 cell line using the baculovirus expression system. Recombinant human FVII (rhFVII) produced in this study carries a 6x-His tag at the C-terminal | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS-PAGE, recombinant fusion protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08709 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | liver cDNA used for cloning | Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
FVII | - |
Homo sapiens |