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Literature summary for 3.4.21.21 extracted from

  • Bjelke, J.R.; Persson, E.; Rasmussen, H.B.; Kragelund, B.B.; Olsen, O.H.
    A loop of coagulation factor VIIa influencing macromolecular substrate specificity (2007), FEBS Lett., 581, 71-76.
    View publication on PubMed
Search for more literature for 3.4.21.21

Activating Compound

Activating Compound Comment Organism Structure
tissue factor TF, complex formation with factor VIIa, structure, overview Homo sapiens

Protein Variants

Protein Variants Comment Organism
E154R site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
E296R site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
K192Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
K341Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
M156K site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
M156Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
M298K site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
M298Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
Q143R site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
Q176G site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
Q286R site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
Q40G site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
T151Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
T293Q site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
V158E site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens
V21E site-directed mutagenesis, activity and active site structure in comparison to the wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ binding structure Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
factor IX + H2O Homo sapiens
-
 factor IXa + ?
-
 ?
Factor X + H2O Homo sapiens
-
 Factor Xa + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity of wild-type and mutant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide + H2O chromogenic S-2765 Homo sapiens ?
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O chromogenic substrate S-2288 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
factor IX + H2O
-
 Homo sapiens factor IXa + ?
-
 ?
Factor X + H2O
-
 Homo sapiens Factor Xa + ?
-
 ?
Factor X + H2O macromolecular substrate interactions at exosites, sites within the FVIIa-TF complex distant from the active site of FVIIa, determine affinity and specificity in the productive recognition of FX Homo sapiens Factor Xa + ?
-
 ?
additional information a loop of factor VIIa influences the macromolecular substrate specificity, overview Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
FVIIa
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens