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Literature summary for 3.4.21.21 extracted from

  • Larsen, K.S.; Östergaard, H.; Bjelke, J.R.; Olsen, O.H.; Rasmussen, H.B.; Christensen, L.; Kragelund, B.B.; Stennicke, H.R.
    Engineering the substrate and inhibitor specificities of human coagulation factor VIIa (2007), Biochem. J., 405, 429-438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.21

Crystallization (Commentary)

Crystallization (Comment) Organism
purified complex between Trp-Tyr-Thr-Arg-cmk-FVIIa and sTF1?209, hanging drop vapour diffusion method, 10 mg/ml protein in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl and 2 mM CaCl2, with 0.1 M sodium citrate, 16.5–15.5% w/v PEG 4000 and 12% v/v propan-1-ol, pH 5.6, as the precipitating agent, X-ray diffraction structure determination and analysis, modeling Homo sapiens

Protein Variants

Protein Variants Comment Organism
T239A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
T239G site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
T239I site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens
T239Y site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
antithrombin III complete inhibition Homo sapiens
Trp-Tyr-Thr-Arg-chloromethyl ketone
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of wild-type and mutant enzymes with different substrates, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 catalytic efficiencies of wild-type and mutant enzymes with different substrates, overview Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl 4-guanidinobenzoate + H2O
-
 Homo sapiens ?
-
 ?
benzyloxycarbonyl-D-Arg-Gly-Arg-4-nitroanilide + H2O chromogenic S-2765 Homo sapiens ?
-
 ?
D-Ile-Pro-Arg-4-nitroanilide + H2O chromogenic substrate S-2288 Homo sapiens D-Ile-Pro-Arg + 4-nitroaniline
-
 ?
Factor X + H2O FX cleavage site Asn-Leu-Thr-Ar-/-Ile-Val-Gly-Gly Homo sapiens Factor Xa + ?
-
 ?
additional information substrate and cleavage site specificity with 7-amino-4-carbamoylmethylcoumarin-linked tetrapeptides, specificity profiling of the recombinant enzyme, overview Homo sapiens ?
-
 ?
Trp-Ala-Thr-Arg-7-amido-4-carbamoylmethylcoumarin + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
factor VIIa
-
 Homo sapiens
FVIIa
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics of wild-type and mutant enzymes, overview Homo sapiens