Literature summary for 3.4.21.21 extracted from

Persson, E.; Bak, H.; Ostergaard, A.; Olsen, O.H.
Augmented intrinsic activity of Factor VIIa by replacement of residues 305, 314, 337 and 374: evidence of two unique mutational mechanisms of activity enhancement (2004), Biochem. J., 379, 497-503.

Search for more literature for 3.4.21.21

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in baby hamster kidney cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
antithrombin	0.1 mg/ml in the presence of 1 unit/ml heparin, less than 10% residual activity after 15min incubation with enzyme FVIIa-sTF	Homo sapiens
p-aminobenzamidine	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0018	-	Factor X	mutant FVIIaIIa	Homo sapiens
0.0021	-	Factor X	mutant FVIIaIIa-sTF	Homo sapiens
0.0021	-	Factor X	mutant FVIIaVEAY-sTF	Homo sapiens
0.0027	-	Factor X	mutant FVIIaVEAY	Homo sapiens
0.003	-	Factor X	wild type FVIIa-sTF	Homo sapiens
0.0044	-	Factor X	wild type FVIIa	Homo sapiens
0.85	-	D-Ile-Pro-Arg-p-nitroanilide	mutant FVIIaVEAY-sTF	Homo sapiens
1.6	-	D-Ile-Pro-Arg-p-nitroanilide	wild type FVIIa-sTF	Homo sapiens
1.7	-	D-Ile-Pro-Arg-p-nitroanilide	mutant FVIIaIIa-sTF	Homo sapiens
1.9	-	D-Ile-Pro-Arg-p-nitroanilide	mutant FVIIaVEAY	Homo sapiens
6.7	-	D-Ile-Pro-Arg-p-nitroanilide	mutant FVIIaIIa	Homo sapiens
10.4	-	D-Ile-Pro-Arg-p-nitroanilide	wild type FVIIa	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
method not mentioned	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine-p-nitroanilide + H2O	-	Homo sapiens	p-nitroaniline + benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine	-	?
D-Ile-Pro-Arg-p-nitroanilide + H2O	-	Homo sapiens	p-nitroaniline + D-Ile-Pro-Arg	-	?
D-Phe-pipecolyl-Arg-p-nitroanilide + H2O	-	Homo sapiens	D-Phe-pipecolyl-Arg + p-nitroaniline	-	?
factor X + H2O	-	Homo sapiens	factor Xa	-	?
pyroGlu-Pro-Arg-p-nitroanilide + H2O	-	Homo sapiens	p-nitroaniline + pyroGlu-Pro-Arg	-	?

Synonyms

Synonyms	Comment	Organism
FVIIa	-	Homo sapiens
FVIIa-sTF	FVIIa in complex with soluble tissue factor	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00015	-	Factor X	wild type FVIIa	Homo sapiens
0.0012	-	Factor X	mutant FVIIaVEAY	Homo sapiens
0.0053	-	Factor X	mutant FVIIaIIa	Homo sapiens
0.007	-	Factor X	mutant FVIIaVEAY-sTF	Homo sapiens
0.0077	-	Factor X	wild type FVIIa-sTF	Homo sapiens
0.024	-	Factor X	mutant FVIIaIIa-sTF	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	because of competitive binding of p-aminobenzamidine and D-Ile-Pro-Arg-p-nitroanilide, the apparent Ki values were calculated from the p-aminobenzamidine IC50, taking into account the concentration of D-Ile-Pro-Arg-p-nitroanilide ([S]) and the Km for this substrate using Ki =IC50/(1 + [S]/Km)	Homo sapiens
0.038	-	p-aminobenzamidine	mutant FVIIaVEAY-sTF, 210 nM	Homo sapiens
0.05	-	p-aminobenzamidine	mutant FVIIaIIa-sTF, 210 nM	Homo sapiens
0.077	-	p-aminobenzamidine	wild type FVIIa-sTF, 210 nM	Homo sapiens
0.177	-	p-aminobenzamidine	mutant FVIIaVEAY, 10 nM	Homo sapiens
0.226	-	p-aminobenzamidine	mutant FVIIaIIa, 25 nM	Homo sapiens
1.41	-	p-aminobenzamidine	wild type FVIIa, 100 nM	Homo sapiens

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Release 2023.1 (January 2023)