Protein Variants | Comment | Organism |
---|---|---|
K305V | the ratio of turnover number to Km-value is 5.3fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
K337A | the ratio of turnover number to Km-value is 4.4fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
L305V/K337A | the ratio of turnover number to Km-value is 6.3fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
M298Q | the ratio of turnover number to Km-value is 6.9fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
V158D/D296V/M298Q | the ratio of turnover number to Km-value is 37.5fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
V158D/D296V/M298Q/K337A | the ratio of turnover number to Km-value is 56.3fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
V158D/D296V/M298Q/L305V | the ratio of turnover number to Km-value is 50fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
V158D/D296V/M298Q/L305V/L337 | the ratio of turnover number to Km-value is 100fold higher than that of the wild-type enzyme with factor X as substrate, increased inhibition rate compared to wild-type enzyme with antithrobin in presence of heparin | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antithrombin | in presence of heparin, 20% loss of activity of wild-type enzyme after 15 min, 59% loss of activity of the K337A mutant enzyme after 15 min, 77% loss of activity of the L305V/K337A mutant enzyme after 15 min, 78% loss of activity of the V158D/D296V/M298Q mutant enzyme after 15 min, 87% loss of activity of the V158D/D296V/M298Q/K337A mutant enzyme after 15 min, 91% loss of activity of the V158D/D296V/M298Q/L305V/K337A mutant enzyme after 15 min, 53% loss of activity of the M298Q mutant enzyme after 15 min | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
Factor X | pH 7.4, 37°C, mutant enzyme L305V/K337A | Homo sapiens | |
0.002 | - |
Factor X | pH 7.4, 37°C, mutant enzyme K337A | Homo sapiens | |
0.0021 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V/L337 | Homo sapiens | |
0.0023 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q | Homo sapiens | |
0.0024 | - |
Factor X | pH 7.4, 37°C, mutant enzyme M298Q | Homo sapiens | |
0.0024 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/K337A | Homo sapiens | |
0.0027 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V | Homo sapiens | |
0.0029 | - |
Factor X | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
0.0029 | - |
Factor X | pH 7.4, 37°C, mutant enzyme K305V | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ile-Pro-Arg-p-nitroanilide + H2O | i.e. S2288 | Homo sapiens | D-Ile-Pro-Arg + p-nitroaniline | - |
? | |
factor X + H2O | - |
Homo sapiens | activated factor X + ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
94 | - |
Factor X | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
280 | - |
Factor X | pH 7.4, 37°C, mutant enzyme K337A | Homo sapiens | |
370 | - |
Factor X | pH 7.4, 37°C, mutant enzyme L305V/K337A | Homo sapiens | |
480 | - |
Factor X | pH 7.4, 37°C, mutant enzyme K305V | Homo sapiens | |
520 | - |
Factor X | pH 7.4, 37°C, mutant enzyme M298Q | Homo sapiens | |
2600 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q | Homo sapiens | |
4200 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V | Homo sapiens | |
4400 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/K337A | Homo sapiens | |
6800 | - |
Factor X | pH 7.4, 37°C, mutant enzyme V158D/D296V/M298Q/L305V/L337 | Homo sapiens |