Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin + H2O | Homo sapiens | - |
fragments of fibronectin | - |
? | |
additional information | Homo sapiens | The enzyme has a dual specificity consisting of chymase and tryptase-type activities. Phe, Tyr, Trp and Leu are preferred in the P1 position. The enzyme has a preference for negatively charged amino acids in the P2A position of substrates and a preference for aliphatic amino acids both upstream and downstream of the cleavage site | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neutrophil | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fibronectin + H2O | - |
Homo sapiens | fragments of fibronectin | - |
? | |
additional information | The enzyme has a dual specificity consisting of chymase and tryptase-type activities. Phe, Tyr, Trp and Leu are preferred in the P1 position. The enzyme has a preference for negatively charged amino acids in the P2A position of substrates and a preference for aliphatic amino acids both upstream and downstream of the cleavage site | Homo sapiens | ? | - |
? | |
additional information | no activity with succinyl-AAPI-4-nitroanilide, succinyl-AAPA-4-nitroanilide, succinyl-AAPV-4-nitroanilide, succinyl-VLGR-4-nitroanilide, Z-GPR-4-nitroanilide, acetyl-YVAD-4-nitroanilide, acetyl-VEID-4-nitroanilide, and acetyl-IEPD-4-nitroanilide | Homo sapiens | ? | - |
? | |
succinyl-AAPF-4-nitroanilide + H2O | - |
Homo sapiens | succinyl-AAPF + 4-nitroaniline | - |
? | |
succinyl-AAPL-4-nitroanilide + H2O | - |
Homo sapiens | succinyl-AAPL + 4-nitroaniline | - |
? | |
succinyl-LLVY-4-nitroanilide + H2O | - |
Homo sapiens | succinyl-LLVY + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Homo sapiens |