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Literature summary for 3.4.21.20 extracted from
- Isolation and some physical and chemical properties of elastase and cathepsin G from dog neutrophils (2001), Biochemistry (Moscow), 66, 1008-1013.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by EDTA and leupeptin both at 0.1 mM | Canis lupus familiaris | |
| pepstatin | 15% inhibition after 30 min at 0.1 mM at 25°C | Canis lupus familiaris |  |
| PMSF | complete inhibition at 0.2 mM after 20 h at 25°C | Canis lupus familiaris | |
| Soybean trypsin inhibitor | complete inhibition at 0.2 mM after 30 min at 25°C | Canis lupus familiaris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.42 | - |
N-benzoyl-L-tyrosine ethyl ester | pH 7.0-9.0, 25°C | Canis lupus familiaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
gel filtration | Canis lupus familiaris |
| 25500 | - |
x * 25500, SDS-PAGE | Canis lupus familiaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Canis lupus familiaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity from leukocytes, 108.5fold | Canis lupus familiaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leukocyte | - |
Canis lupus familiaris | - |
| neutrophil | - |
Canis lupus familiaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.51 | - |
purified enzyme | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-benzoyl-L-tyrosine ethyl ester + H2O | - |
Canis lupus familiaris | N-benzoyl-L-tyrosine + ethanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25500, SDS-PAGE | Canis lupus familiaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Canis lupus familiaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Canis lupus familiaris |
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Release 2023.1 (January 2023)
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