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Literature summary for 3.4.21.19 extracted from

  • Leshchinskaya, L.B.; Shakirov, E.V.; Itskovitch, E.L.; Balaban, N.P.; Mardanova, A.M.; Sharipova, M.R.; Viryasov, M.B.; Rudenskaya, G.N.; Stepanov, V.M.
    Glutamyl endopeptidase of Bacillus intermedius, strain 3-19 (1997), FEBS Lett., 404, 241-244.
    View publication on PubMed
Search for more literature for 3.4.21.19

General Stability

General Stability Organism
Ca2+ stabilizes Bacillus intermedius

Inhibitors

Inhibitors Comment Organism Structure
diisopropyl fluorophosphate
-
 Bacillus intermedius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6
-
 benzyloxycarbonyl-Glu-p-nitroanilide
-
 Bacillus intermedius

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, doubles activity Bacillus intermedius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Bacillus intermedius

Organism

Organism UniProt Comment Textmining
Bacillus intermedius
-
 strain 3-19
-
Bacillus intermedius Mrz 19
-
 strain 3-19
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus intermedius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Lys-Asp-Val-Tyr + H2O only 5.6% hydrolysis of the Asp-Val bond Bacillus intermedius Arg-Lys-Asp + Val-Tyr
-
 ?
Arg-Lys-Glu-Val-Tyr + H2O completely splits Glu-Val bond, after 30 min Bacillus intermedius Arg-Lys-Glu + Val-Tyr
-
 ?
Arg-Lys-Glu-Val-Tyr + H2O completely splits Glu-Val bond, after 30 min Bacillus intermedius Mrz 19 Arg-Lys-Glu + Val-Tyr
-
 ?
benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Glu-p-nitroanilide + H2O
-
 Bacillus intermedius Mrz 19 benzyloxycarbonyl-Glu + p-nitroaniline
-
 ?
casein + H2O
-
 Bacillus intermedius ?
-
 ?
casein + H2O
-
 Bacillus intermedius Mrz 19 ?
-
 ?
Glucagon + H2O
-
 Bacillus intermedius ?
-
 ?
Glucagon + H2O
-
 Bacillus intermedius Mrz 19 ?
-
 ?
insulin + H2O A-chain and B-chain Bacillus intermedius ?
-
 ?
insulin + H2O A-chain and B-chain Bacillus intermedius Mrz 19 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Bacillus intermedius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus intermedius
65
-
 in presence of Ca2+ Bacillus intermedius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
 pH 6.5-11.0, 3 h, stable Bacillus intermedius
50
-
 pH 8.0, 24 h, in presence of 5 mM Ca2+, stable Bacillus intermedius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 and a second optimum at pH 9.0, hydrolysis of casein Bacillus intermedius
8
-
 hydrolysis of benzyloxycarbonyl-Glu-p-nitroanilide Bacillus intermedius
9
-
 and a second optimum at pH 7.5, hydrolysis of casein Bacillus intermedius

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11 22°C, 3 h, stable Bacillus intermedius
8
-
 50°C, 24 h, in presence of 5 mM Ca2+, stable Bacillus intermedius