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Literature summary for 3.4.21.19 extracted from

  • Ono, T.; Nemoto, T.K.; Shimoyama, Y.; Kimura, S.; Ohara-Nemoto, Y.
    An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation (2008), Anal. Biochem., 381, 74-80.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Thermolysin
-
Staphylococcus aureus
Thermolysin
-
Staphylococcus epidermidis
Thermolysin
-
Staphylococcus warneri

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus
expressed in Escherichia coli Staphylococcus epidermidis
expressed in Escherichia coli Staphylococcus warneri

Protein Variants

Protein Variants Comment Organism
additional information GluV8mut5-SW, prepro-GluV8mut5 attached to mature GluSW Staphylococcus warneri
additional information GluV8mut5, full-length GluV8 with five substitutions (Asp36His/Glu62Gln/Glu65Ser/Ala67Pro/Asn68Ser) in the prosegment, GluV8mut5DELTAC, GluV8mut5 with C-terminal 52 residues deleted, GluV8mut4nCSer237Ala, GluV8mut4nC with an amino acid substitution (Ser237Ala), GluV8mut5-SW, prepro-GluV8mut5 attached to mature GluSW Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
SDS-PAGE, wild-type GluSW Staphylococcus warneri
38000
-
GluV8mut5-SW, SDS-PAGE Staphylococcus aureus
38000
-
SDS-PAGE , GluV8mut5-SW Staphylococcus warneri
44000
-
SDS-PAGE. GluV8 is scarcely expressed. Mutant GluV8mut5 shows a 44 kDa band having much higher density than that of GluV8mut4 Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus V8
-
-
-
Staphylococcus epidermidis
-
-
-
Staphylococcus warneri
-
JCM 2415
-
Staphylococcus warneri JCM 2415
-
JCM 2415
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus epidermidis
-
Staphylococcus warneri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O the C-terminal tripeptide of the prosequence of GluV8 (i.e., His66-Ala-Asn68) resembles the unprocessed GluV8 cleavable substrate Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide Staphylococcus aureus ?
-
?
Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O the C-terminal tripeptide of the prosequence of GluV8 (i.e., His66-Ala-Asn68) resembles the unprocessed GluV8 cleavable substrate Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide Staphylococcus aureus V8 ?
-
?
Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O GluV8 also possesses trace activity toward Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide that is at least 30fold higher than the activity for residual 4-methylcoumaryl-7-amide peptides that carried Ala, Phe, or Leu at their P1 position Staphylococcus aureus ?
-
?
Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O GluV8 also possesses trace activity toward Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide that is at least 30fold higher than the activity for residual 4-methylcoumaryl-7-amide peptides that carried Ala, Phe, or Leu at their P1 position Staphylococcus aureus V8 ?
-
?
Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O
-
Staphylococcus aureus ?
-
?
Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O
-
Staphylococcus epidermidis ?
-
?
Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O
-
Staphylococcus warneri ?
-
?
Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O
-
Staphylococcus warneri JCM 2415 ?
-
?
Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O
-
Staphylococcus aureus V8 ?
-
?

Synonyms

Synonyms Comment Organism
GluSE
-
Staphylococcus epidermidis
GluSW
-
Staphylococcus warneri
glutamyl endopeptidase
-
Staphylococcus epidermidis
glutamyl endopeptidase
-
Staphylococcus warneri
glutamyl endopeptidase I
-
Staphylococcus aureus
GluV8
-
Staphylococcus aureus
V8 protease
-
Staphylococcus aureus