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Literature summary for 3.4.21.19 extracted from

  • Kawalec, M.; Potempa, J.; Moon, J.L.; Travis, J.; Murray, B.E.
    Molecular diversity of a putative virulence factor: purification and characterization of isoforms of an extracellular serine glutamyl endopeptidase of Enterococcus faecalis with different enzymatic activities (2005), J. Bacteriol., 187, 266-275.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.19

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarin 0.3 mM, 62% inhibition of isoform -1S-SprE Enterococcus faecalis
diisopropyl fluorophosphate 0.3 mM, complete inhibition of isoform -1S-SprE Enterococcus faecalis
additional information not inhibitory: E-64, pepstatin, o-phenanthroline, EDTA Enterococcus faecalis
NaCl 1 mM, 80% inhibition of isoform -1S-SprE Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.027
-
 succinyl-Ala-Ala-Pro-Glu-p-nitroanilide isoform -1S-SprE, pH 7.5, 37°C Enterococcus faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Enterococcus faecalis
-
-

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis Q47809
-
-
Enterococcus faecalis OG1RF Q47809
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification proteolytic modification of zymogen dependent on the presence of gelatinase. In absence of gelatinase, maturation to different isoforms including a highly active isoform -1S-SprE with protein sequence Ser-1 to Glu227 Enterococcus faecalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20140
-
 isoform -1S-SprE, pH 7.5, 37°C Enterococcus faecalis

Storage Stability

Storage Stability Organism
-70°C, all isoforms stable for at least 6 months Enterococcus faecalis
4°C, isoform -1S-SprE, 50% loss of activity within 4 weeks, isoform 1l-SprE, stable Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O 35% of the efficiency with succinyl-Ala-Ala-Pro-Glu-p-nitroanilide Enterococcus faecalis benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O 35% of the efficiency with succinyl-Ala-Ala-Pro-Glu-p-nitroanilide Enterococcus faecalis OG1RF benzyloxycarbonyl-Phe-Leu-Glu + p-nitroaniline
-
 ?
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide + H2O
-
 Enterococcus faecalis succinyl-Ala-Ala-Pro-Glu + p-nitroaniline
-
 ?
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide + H2O
-
 Enterococcus faecalis OG1RF succinyl-Ala-Ala-Pro-Glu + p-nitroaniline
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 isoform -1S-SprE, inversely dependent on the ionic strength of buffer Enterococcus faecalis