Cloned (Comment) | Organism |
---|---|
expression of recombinant enzymes in Escherichia coli | Lysobacter enzymogenes |
Crystallization (Comment) | Organism |
---|---|
- |
Lysobacter enzymogenes |
Protein Variants | Comment | Organism |
---|---|---|
G216A | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216D | no active enzyme | Lysobacter enzymogenes |
G216F | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216G | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216H | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216I | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216K | no active enzyme | Lysobacter enzymogenes |
G216L | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216N | no active enzyme | Lysobacter enzymogenes |
G216P | no active enzyme | Lysobacter enzymogenes |
G216Q | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216R | no active enzyme | Lysobacter enzymogenes |
G216S | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216T | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216V | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216W | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
G216Y | active enzyme, but production levels for the mutants with larger substitutions do decrease significantly | Lysobacter enzymogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Lysobacter enzymogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lysobacter enzymogenes | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-Ala-Ala-Pro-X-p-nitroanilide + H2O | X: Gly, Thr, Val, Leu, Ile, Met, Phe | Lysobacter enzymogenes | ? | - |
? |