Activating Compound | Comment | Organism | Structure |
---|---|---|---|
chondroitin | - |
Homo sapiens | |
dermatan sulfate | - |
Homo sapiens | |
enterokinase | - |
Homo sapiens | |
Sodium citrate | - |
Homo sapiens | |
Sodium sulfate | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in a baculovirus/insect cell line system | Homo sapiens |
General Stability | Organism |
---|---|
KLK13 activity is reduced close to zero in the presence of 50 mM Tris HCl (with no sodium citrate or heparin) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
0.0022 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
0.0053 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
0.031 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cervix | - |
Homo sapiens | - |
esophagus | - |
Homo sapiens | - |
salivary gland | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
tonsil | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-GFSPFRSSRIQ-EDDnp + H2O | - |
Homo sapiens | Abz-GFSPFR + SSRIQ-EDDnp | - |
? | |
Abz-KLKSSKQ-EDDnp + H2O | - |
Homo sapiens | Abz-KLK + SSKQ-EDDnp | - |
? | |
Abz-KLRKSKQ-EDDnp + H2O | - |
Homo sapiens | Abz-KLR + KSKQ-EDDnp | - |
? | |
Abz-KLRRSKQ-EDDnp + H2O | - |
Homo sapiens | Abz-KLR + RSKQ-EDDnp | - |
? | |
Abz-KLRSSKQ-EDDnp + H2O | best substrate | Homo sapiens | Abz-KLR + SSKQ-EDDnp | - |
? | |
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O | rapid cleavage | Homo sapiens | Abz-MISLMKRPPGFSPFR + SSRI-NH2 | - |
? | |
Abz-MISLMKRPQ-EDDnp + H2O | - |
Homo sapiens | Abz-MISLMK + RPQ-EDDnp | - |
? | |
histatin 3 + H2O | i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate | Homo sapiens | DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN | - |
? | |
low molecular weight human kininogen + H2O | - |
Homo sapiens | ? | - |
? | |
additional information | KLK13 does not hydrolyze Abz-KLRESKQ-EDDnp, Abz-KLRSEKQ-EDDnp, and AbzeELRSSKQ-EDDnp | Homo sapiens | ? | - |
? | |
additional information | the S2' subsite of KLK13 does not have restricted specificity but does present preference for the basic amino acid R and does not accepted negative charge | Homo sapiens | ? | - |
? | |
myelin basic protein + H2O | at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 30000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
kallikrein-related peptidase 13 | - |
Homo sapiens |
KLK13 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
0.5 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
3.2 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
3.4 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | KLK13 plays a role in the defense of the upper digestive apparatus and in male reproductive organs | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
19 | - |
Abz-KLKSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
1455 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens | |
2834 | - |
Abz-KLRSSKQ-EDDnp | in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C | Homo sapiens |