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Literature summary for 3.4.21.119 extracted from

  • Andrade, D.; Assis, D.M.; Santos, J.A.; Alves, F.M.; Hirata, I.Y.; Araujo, M.S.; Blaber, S.I.; Blaber, M.; Juliano, M.A.; Juliano, L.
    Substrate specificity of kallikrein-related peptidase 13 activated by salts or glycosaminoglycans and a search for natural substrate candidates (2011), Biochimie, 93, 1701-1709.
    View publication on PubMed
Search for more literature for 3.4.21.119

Activating Compound

Activating Compound Comment Organism Structure
chondroitin
-
 Homo sapiens
dermatan sulfate
-
 Homo sapiens
enterokinase
-
 Homo sapiens
Sodium citrate
-
 Homo sapiens
Sodium sulfate
-
 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in a baculovirus/insect cell line system Homo sapiens

General Stability

General Stability Organism
KLK13 activity is reduced close to zero in the presence of 50 mM Tris HCl (with no sodium citrate or heparin) Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
0.0022
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
0.0053
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
0.031
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cervix
-
 Homo sapiens
-
esophagus
-
 Homo sapiens
-
salivary gland
-
 Homo sapiens
-
testis
-
 Homo sapiens
-
tonsil
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-GFSPFRSSRIQ-EDDnp + H2O
-
 Homo sapiens Abz-GFSPFR + SSRIQ-EDDnp
-
 ?
Abz-KLKSSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLK + SSKQ-EDDnp
-
 ?
Abz-KLRKSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLR + KSKQ-EDDnp
-
 ?
Abz-KLRRSKQ-EDDnp + H2O
-
 Homo sapiens Abz-KLR + RSKQ-EDDnp
-
 ?
Abz-KLRSSKQ-EDDnp + H2O best substrate Homo sapiens Abz-KLR + SSKQ-EDDnp
-
 ?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O rapid cleavage Homo sapiens Abz-MISLMKRPPGFSPFR + SSRI-NH2
-
 ?
Abz-MISLMKRPQ-EDDnp + H2O
-
 Homo sapiens Abz-MISLMK + RPQ-EDDnp
-
 ?
histatin 3 + H2O i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate Homo sapiens DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN
-
 ?
low molecular weight human kininogen + H2O
-
 Homo sapiens ?
-
 ?
additional information KLK13 does not hydrolyze Abz-KLRESKQ-EDDnp, Abz-KLRSEKQ-EDDnp, and AbzeELRSSKQ-EDDnp Homo sapiens ?
-
 ?
additional information the S2' subsite of KLK13 does not have restricted specificity but does present preference for the basic amino acid R and does not accepted negative charge Homo sapiens ?
-
 ?
myelin basic protein + H2O at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * about 30000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
kallikrein-related peptidase 13
-
 Homo sapiens
KLK13
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
0.5
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.2
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
3.4
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens

General Information

General Information Comment Organism
physiological function KLK13 plays a role in the defense of the upper digestive apparatus and in male reproductive organs Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16
-
 Abz-KLKSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
19
-
 Abz-KLKSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
1455
-
 Abz-KLRSSKQ-EDDnp in the presence of 0.0005 mM heparin, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens
2834
-
 Abz-KLRSSKQ-EDDnp in the presence of 1.4 M sodium citrate, in 50 mM Tris, 1 mM EDTA, pH 7.5, at 37°C Homo sapiens