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Literature summary for 3.4.21.118 extracted from

  • Kishi, T.; Cloutier, S.M.; Kuendig, C.; Deperthes, D.; Diamandis, E.P.
    Activation and enzymatic characterization of recombinant human kallikrein 8 (2006), Biol. Chem., 387, 723-731.
    View publication on PubMed
Search for more literature for 3.4.21.118

Inhibitors

Inhibitors Comment Organism Structure
antipain 50% inhibition at 0.00046 mM Homo sapiens
chymostatin 50% inhibition at 0.008 mM Homo sapiens
leupeptin 50% inhibition at 0.066 mM Homo sapiens
Ni2+ 50% inhibition at 0.011 mM Homo sapiens
Zn2+ 50% inhibition at 0.0033 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 4fold stimulation Homo sapiens
K+ 1.7fold stimulation Homo sapiens
Mg2+ 3foldstimulation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60259 recombinant enzyme
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification pro-enzyme may be activiated by lysyl-endopeptidase by cleavage of a 9 amino acid propeptide Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Gly-Gly-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 1000 M/s Homo sapiens benzyloxycarbonyl-Gly-Gly-L-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 1000 M/s Homo sapiens benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
additional information enzyme shows trypsin-like specificity. Synthetic substrates containing either Arg or Lys at P1 positions are cleaved Homo sapiens ?
-
 ?
t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 21000 M/s Homo sapiens t-butyloxycarbonyl-L-Val-L-Pro-L-Arg + 7-amino-4-methylcoumarin
-
 ?
tosyl-Gly-L-Pro-L-Lys-7-amido-4-methylcoumarin + H2O ratio kcat/KM-value is 670 M/s Homo sapiens tosyl-Gly-L-Pro-L-Lys + 7-amino-4-methylcoumarin
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.5
-
 no detectable activity below Homo sapiens
7.4 10 more than 50% of maximum activity within this range Homo sapiens