Literature summary for 3.4.21.118 extracted from

Kishi, T.; Kato, M.; Shimizu, T.; Kato, K.; Matsumoto, K.; Yoshida, S.; Shiosaka, S.; Hakoshima, T.
Crystal structure of neuropsin, a hippocampal protease involved in kindling epileptogenesis (1999), J. Biol. Chem., 274, 4220-4224.

Search for more literature for 3.4.21.118

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of proneuropsin in Spodoptera frugiperda insect cells via baculovirus infection	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, recombinant enzyme, protein solution: 24.5 mg/ml, 5 mM HEPES, pH 7.4, 100 mM NaCl, 4°C, precipitant with PEG, X-ray diffraction structure determination and analysis	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
polypeptide + H2O	Mus musculus	-	peptides	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q61955	Protein data bank: 1NPM, for crystal structure	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	enzyme has a putative N-glycoslyation site at Asn95 of the kallikrein loop	Mus musculus

Purification (Commentary)

Purification (Comment)	Organism
recombinant from SF9 insect cells	Mus musculus

Reaction

Reaction	Comment	Organism	Reaction ID
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys	enzyme is a trypsin-type serine protease	Mus musculus	a
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys	substrate binding site structure defining the specificity for Arg and Lys in the S1 pocket	Mus musculus	a
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys	the ordered kallikrein loop projects proline towards the active site to restrict smaller residues or proline at the P2 position of substrates	Mus musculus	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Mus musculus	-
hippocampus	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
polypeptide + H2O	-	Mus musculus	peptides	-	?

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)