Application | Comment | Organism |
---|---|---|
diagnostics | the enzyme is a potential biomarker of ovarian cancer | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and in a baculovirus-insect cell system | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
kallikrein 7 bound to inhibitors succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone and Ala-Ala-Phe-chloromethyl ketone, sitting-drop vapor diffusion method at 18°C, 0.001 ml protein solution, containing 7 mg/ml enzyme-inhibitor complex, is mixed with 0.001 ml reservoir solution containing 100 mM [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)-methane-HCl, pH 6.0, 25% PEG 3350, and 2.5 M Li2SO4, equilibration against reservoir solution, X-ray diffraction structure determination and analysis at 1.0-2.5 A resolution | Homo sapiens |
sitting drop vapour diffusion method, with 100 mM [bis(2-hydroxyethyl)amino]tris(hydroxymethyl)-methane-HCl, pH 6.0, 25% PEG 3350, and 2.5 M Li2SO4 or with 100 mM sodium cacodylate, pH 6.5, 200 mM magnesium acetate, and 30% (v/v) 2-methyl-2,4-pentanediol | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H41F | mutant is inhibited by Zn2+ as the wild type enzyme | Homo sapiens |
H41F | site-directed mutagenesis, the mutant is inhibited by Zn2+ like the wild-type enzyme | Homo sapiens |
H99A | mutant exhibits virtually no Zn2+ inhibition | Homo sapiens |
H99A | site-directed mutagenesis, the mutant shows resistance against inhibition by Zn2+ in contrast to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Ala-Phe-chloromethyl ketone | - |
Homo sapiens | |
Ala-Ala-Phe-chloromethylketone | - |
Homo sapiens | |
Cu2+ | inhibition at low micromolar concentrations, structural basis for inhibition, overview; strong inhibitory effect | Homo sapiens | |
succinyl-Ala-Ala-Pro-Phe-chloromethyl ketone | - |
Homo sapiens | |
Zn2+ | inhibition at low micromolar concentrations, noncompetitive inhibition type, structural basis for inhibition involving His99, overview; strong inhibitory effect | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0402 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 25°C | Homo sapiens | |
0.0402 | - |
L-Tyr-7-amido-4-methylcoumarin | in 50 mM HEPES, pH 7.5, 150 mM NaCl, 0.005% Tween 20, at 25°C | Homo sapiens | |
0.0443 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 25°C | Homo sapiens | |
0.0443 | - |
L-Phe-7-amido-4-methylcoumarin | in 50 mM HEPES, pH 7.5, 150 mM NaCl, 0.005% Tween 20, at 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Homo sapiens | |
Ca2+ | slight enhancement of activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49862 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | pro-kallikrein is activated by proteolytic clavage of the Xaa15-Ile16 bond | Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermis | stronlgy expressed in the upper layers of the epidermis | Homo sapiens | - |
epidermis | the enzyme is strongly expressed in the upper layers of the epidermis | Homo sapiens | - |
ovary cancer cell | - |
Homo sapiens | - |
stratum corneum | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-Phe + 7-amino-4-methylcoumarin | - |
? | |
L-Tyr-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | L-Tyr + 7-amino-4-methylcoumarin | - |
? | |
additional information | the enzyme participates in skin desquamation but is also implicated in diverse skin diseases and is a potential biomarker of ovarian cancer | Homo sapiens | ? | - |
? | |
additional information | active site structure and substrate specificity, overview, kallikrein 7 exhibits large positively charged surface patches, representing putative exosites for prime side substrate recognition, overview | Homo sapiens | ? | - |
? | |
Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Phe + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | succinyl-Ala-Ala-Pro-Phe + 7-amino-4-methylcoumarin | - |
? | |
succinyl-Ala-Val-Pro-Phe 4-nitroanilide + H2O | - |
Homo sapiens | succinyl-Ala-Val-Pro-Phe + 4-nitroaniline | - |
? | |
succinyl-Leu-Leu-Tyr-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | succinyl-Leu-Leu-Tyr + 7-amino-4-methylcoumarin | - |
? | |
Tyr-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Tyr + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | tertiary and primary structure of kallikrein 7, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
hK7 | - |
Homo sapiens |
human stratum corneum chymotryptic enzyme | - |
Homo sapiens |
KLK7 | - |
Homo sapiens |
More | the enzyme belongs to the human tissue kallikrein serine proteinase family | Homo sapiens |
tissue kallikrein 7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000016 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 25°C | Homo sapiens | |
0.000016 | - |
L-Phe-7-amido-4-methylcoumarin | in 50 mM HEPES, pH 7.5, 150 mM NaCl, 0.005% Tween 20, at 25°C | Homo sapiens | |
0.000033 | - |
Phe-7-amido-4-methylcoumarin | pH 7.5, 25°C | Homo sapiens | |
0.000033 | - |
L-Tyr-7-amido-4-methylcoumarin | in 50 mM HEPES, pH 7.5, 150 mM NaCl, 0.005% Tween 20, at 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
Cu2+ | apparent value | Homo sapiens | |
0.0006 | - |
Cu2+ | 25°C | Homo sapiens | |
0.01 | - |
Zn2+ | apparent value | Homo sapiens | |
0.01 | - |
Zn2+ | 25°C | Homo sapiens |