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Literature summary for 3.4.21.117 extracted from

  • Debela, M.; Magdolen, V.; Schechter, N.; Valachova, M.; Lottspeich, F.; Craik, C.S.; Choe, Y.; Bode, W.; Goettig, P.
    Specificity profiling of seven human tissue kallikreins reveals individual subsite preferences (2006), J. Biol. Chem., 281, 25678-25688.
    View publication on PubMed
Search for more literature for 3.4.21.117

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15(pREP4) cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P49862 precursor
-

Purification (Commentary)

Purification (Comment) Organism
nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-FR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-FR + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-LR-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-LR + 7-amino-4-methylcoumarin
-
 ?
methoxy-succinyl-Arg-Pro-Tyr-p-nitroanilide + H2O
-
 Homo sapiens methoxy-succinyl-Arg-Pro-Tyr + p-nitroaniline
-
 ?
additional information succinyl-Ala-Ala-Pro-Phe-tripeptidyl-p-nitroanilide is no substrate Homo sapiens ?
-
 ?
additional information the most favored P1 residue of hK7 is Tyr followed by Ala, Met, and Nle, whereas Phe is ranked rather low, and Trp seems to be totally excluded, at S2, the most preferred residue is again Tyr, well ahead of the medium-sized hydrophobic residues Leu, Nle, Thr, Met, and Phe, whereas Gly is almost not tolerated, at S3 and S4, nearly all residues are accepted with a slight preference for hydrophobic side chains Homo sapiens ?
-
 ?
succinyl-AAPF-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens succinyl-AAPF + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
hK7 exhibits a chymotrypsin-like specificity preferring large hydrophobic or polar residues at the P1 position Homo sapiens
human stratum corneum chymotryptic enzyme
-
 Homo sapiens