Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.21.116 extracted from

  • Zhou, R.; Kroos, L.
    BofA protein inhibits intramembrane proteolysis of pro-sigmaK in an intercompartmental signaling pathway during Bacillus subtilis sporulation (2004), Proc. Natl. Acad. Sci. USA, 101, 6385-6390.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.116

Protein Variants

Protein Variants Comment Organism
additional information coexpression of enzyme and its substrate pro-sigmaK in Escherichia coli. Enzyme shows abundant and accurate cleavage of substrate. Coexpression of Bacillus subtilis protein BofA in this system leads to formation of a complex of enzyme and BofA and marked inhibition of pro-sigmaK processing. Inhibition may occur by providing H57 of BofA as metal ligand to the catalytic center of enzyme Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-sigmaK + H2O Bacillus subtilis
-
 sigmaK + 20 amino acid peptide
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
N-terminal cleavage of the pro-form of the sporulation protein sigmaK enzyme is a metalloprotease with a transmembrane H43EXXH47 motif, E44 promotes nucleophilic attack by a water molecule on the carbonyl atom of the substrate peptide bond Bacillus subtilis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pro-sigmaK + H2O
-
 Bacillus subtilis sigmaK + 20 amino acid peptide
-
 ?
pro-sigmaK + H2O amino acids 1-126 of substrate pro-sigmaK are sufficient to serve as a substrate Bacillus subtilis sigmaK + 20 amino acid peptide
-
 ?