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Literature summary for 3.4.21.116 extracted from

  • Wakeley, P.R.; Dorazi, R.; Hoa, N.T.; Bowyer, J.R.; Cutting, S.M.
    Proteolysis of SpoIVB is a critical determinant in signalling of Pro-sK processing in Bacillus subtilis (2000), Mol. Microbiol., 36, 1336-1348.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
S378A mutant with blocked self-cleavage Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
three predominant species of approximately 45000 Da, 43000 Da and 42000 Da after processing Bacillus subtilis
43000
-
three predominant species of approximately 45000 Da, 43000 Da and 42000 Da after processing Bacillus subtilis
45000
-
three predominant species of approximately 45000 Da, 43000 Da and 42000 Da after processing Bacillus subtilis
52000
-
S378A mutant, unprocessed proprotein Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-sigmaK Bacillus subtilis enzyme is essential for intercompartmental signalling in the sigmaK-checkpoint, activates poteolytic processing of pro-sigmaK to its mature and active form sigmaK, function in formation of heat-resistant spores sigmaK + ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme is able to self-cleave in trans into at least three discrete products, zymogen is subject to two levels of proteolysis: autoproteolysis generating intermediate products of 42000 Da, 43000 Da and 45000 Da, at least one of which is proposed to be the active form, followed by processing by one or more enzymes to smaller species of 42000 Da and 40000 Da Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information self-cleavage occurs at three different sites: Val52 and Asn53, Ala62 and Phe63, Val74 and Thr75 Bacillus subtilis ?
-
?
pro-sigmaK
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Bacillus subtilis sigmaK + ?
-
?
pro-sigmaK enzyme is essential for intercompartmental signalling in the sigmaK-checkpoint, activates poteolytic processing of pro-sigmaK to its mature and active form sigmaK, function in formation of heat-resistant spores Bacillus subtilis sigmaK + ?
-
?

Synonyms

Synonyms Comment Organism
S55.001 Merops ID Bacillus subtilis