Cloned (Comment) | Organism |
---|---|
functional expression of wild-type, and expression of amino acid exchange mutants in the mutant strain BSL51, lacking spoIVFA and spoIVFB genes, and in the mutant OR745, lacking gene spoIVFB | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D137A | mutant shows no remaining sporulation activity | Bacillus subtilis |
D137H | mutant shows no remaining sporulation activity | Bacillus subtilis |
D137N | mutant shows highly reduced sporulation activity | Bacillus subtilis |
E44A | mutant shows nearly no remaining sporulation activity | Bacillus subtilis |
E44D | mutant shows increased sporulation activity | Bacillus subtilis |
E44Q | mutant shows highly reduced sporulation activity | Bacillus subtilis |
H43F | mutant shows no remaining sporulation activity, no immunologically detectable enzyme protein | Bacillus subtilis |
H47F | mutant shows no remaining sporulation activity | Bacillus subtilis |
I42P | mutant shows increased sporulation activity | Bacillus subtilis |
additional information | comparison of effects of spoIVFB mutation with spoIVFB mutation on accumulation level of the enzymes in the recombinant strains, overview | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | belongs to the family of putative membrane metalloproteases | Bacillus subtilis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | Bacillus subtilis | processing of prosigmaK, which is a protein involved in sporulation | sigmaK + 20 amino acid peptide | - |
? | |
pro-sigmaK + H2O | Bacillus subtilis PY79 | processing of prosigmaK, which is a protein involved in sporulation | sigmaK + 20 amino acid peptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P26937 | - |
- |
Bacillus subtilis PY79 | P26937 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
self-cleaves Val52-/-Asn53, Ala62-/-Phe63 and Val74-/-Thr75 at the N-terminus of SpoIVB | enzyme contains conserved metalloprotease sequence motif HEXXH | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-sigmaK + H2O | processing of prosigmaK, which is a protein involved in sporulation | Bacillus subtilis | sigmaK + 20 amino acid peptide | - |
? | |
pro-sigmaK + H2O | processing of prosigmaK, which is a protein involved in sporulation | Bacillus subtilis PY79 | sigmaK + 20 amino acid peptide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M50.002 | Merops-ID | Bacillus subtilis |
SpolVFB | - |
Bacillus subtilis |
stage IV sporulation protein FB | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in vivo assay at | Bacillus subtilis |