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Literature summary for 3.4.21.115 extracted from
- Crystal structure of a viral protease intramolecular acyl-enzyme complex: insights into cis-cleavage at the VP4/VP3 junction of Tellina birnavirus (2011), J. Biol. Chem., 286, 12475-12482.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Tuner (DE3) cells | Tellina virus 1 |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| VP4 with the C-terminus binding into its own active site forming an intramolecular (cis) acyl-enzyme complex, hanging drop vapor diffusion method, using 21% (w/v) PEG8000 and 0.55 M ammonium sulfate | Tellina virus 1 |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24745 | - |
x * 24745, calculated from amino acid sequence | Tellina virus 1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tellina virus 1 | Q2PBR5 | residue 619-830 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, SP-Sepharose column chromatography, and Sephacryl S-100 gel filtration | Tellina virus 1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NH2-pVP2-X-VP4-VP3-COOH + H2O | VP4 is encoded as part of the 1114-residue long segment A polyprotein NH2-pVP2-X-VP4-VP3-COOH. It cleaves after amino acid residue positions 512, 618, and 830 to yield capsid precursor protein pVP2, peptide X, VP4 itself, and ribonucleoprotein VP3. Additional cleavage sites were found at the C-terminal side of amino acids 451, 492, and 499 within the pVP2 region | Tellina virus 1 | pVP2 + peptide X + VP4 + VP3 | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24745, calculated from amino acid sequence | Tellina virus 1 |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| VP4 | - |
Tellina virus 1 |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Tellina virus 1 | calculated from amino acid sequence | - |
9.9 |
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