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Literature summary for 3.4.21.115 extracted from

  • Petit, S.; Lejal, N.; Huet, J.C.; Delmas, B.
    Active residues and viral substrate cleavage sites of the protease of the birnavirus infectious pancreatic necrosis virus (2000), J. Virol., 74, 2057-2066.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.115

Cloned(Commentary)

Cloned (Comment) Organism
wild-type IPNA and truncated forms, expressed in Escherichia coli infectious pancreatic necrosis virus

Protein Variants

Protein Variants Comment Organism
D537Q mutant with wild-type activity infectious pancreatic necrosis virus
D585I mutant with wild-type activity infectious pancreatic necrosis virus
D595L mutant with wild-type activity infectious pancreatic necrosis virus
D601S mutant with wild-type activity infectious pancreatic necrosis virus
D644I mutant with wild-type activity infectious pancreatic necrosis virus
D660G/D661S mutant with wild-type activity infectious pancreatic necrosis virus
D672N mutant with wild-type activity infectious pancreatic necrosis virus
D693L reduced activity for the VP4-VP3 junction infectious pancreatic necrosis virus
H547S reduced activity for the VP4-VP3 junction infectious pancreatic necrosis virus
H697L reduced activity for the VP4-VP3 junction infectious pancreatic necrosis virus
H704S mutant with wild-type activity infectious pancreatic necrosis virus
S633A no enzyme activity infectious pancreatic necrosis virus
S633C 40% reduction of VP2-VP4 cleavage, 80% reduction of VP4-VP3 cleavage infectious pancreatic necrosis virus
S633Q no enzyme activity infectious pancreatic necrosis virus
S633T no enzyme activity infectious pancreatic necrosis virus

Organism

Organism UniProt Comment Textmining
infectious pancreatic necrosis virus Q703G9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification self-cleavage of IPNV VP4 at the pVP2-VP4 and VP4-VP3 junctions infectious pancreatic necrosis virus

Purification (Commentary)

Purification (Comment) Organism
-
 infectious pancreatic necrosis virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polyprotein of IPNV + H2O cleaves at the (Ser/Thr)-X-Ala-/-(Ser/Ala)-Gly motif, -/- = cleavage site infectious pancreatic necrosis virus pVP2 + VP3 + VP4
-
 ?

Synonyms

Synonyms Comment Organism
NS
-
 infectious pancreatic necrosis virus
S50.001 Merops ID infectious pancreatic necrosis virus
VP4
-
 infectious pancreatic necrosis virus