Literature summary for 3.4.21.112 extracted from

Bodvard, K.; Mohlin, J.; Knecht, W.
Recombinant expression, purification, and kinetic and inhibitor characterisation of human site-1-protease (2007), Protein Expr. Purif., 51, 308-319.

Search for more literature for 3.4.21.112

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	increase in activity about 40% at 10 mM	Homo sapiens
EGTA	increase in activity about 40% at 10 mM	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
first 997 amino acids of human S1P lacking the C-terminal transmembrane region. Full-length sequence of human S1P. Both tagged with a C-terminal 8 x His-tag, subcloned into the entry vector pDONR201 and expressed by baculovirus expression vector system in Sf9 cells. Additionally, first 997 amino acids of human S1P lacking the C-terminal transmembrane region expressed in Sf21 cells and Trichoplusia ni egg, High Five cells	Homo sapiens

Cloned (Comment)

Organism

first 997 amino acids of human S1P lacking the C-terminal transmembrane region. Full-length sequence of human S1P. Both tagged with a C-terminal 8 x His-tag, subcloned into the entry vector pDONR201 and expressed by baculovirus expression vector system in Sf9 cells. Additionally, first 997 amino acids of human S1P lacking the C-terminal transmembrane region expressed in Sf21 cells and Trichoplusia ni egg, High Five cells

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
3,4-dichloroisocoumarin	potent slow binding inhibitor, 100% inhibition by 0.05 mM	Homo sapiens
Co2+	-	Homo sapiens
decanoyl-RVKR-chlorometylketone	66% inhibition at 0.05 mM	Homo sapiens
gabexate mesylate	8% inhibition at 0.05 mM	Homo sapiens
additional information	not inhibited by Mn2+, 7B2 C-terminal peptide, ProSAAS235-244 peptide, ProSAAS235-246 peptide, trypsin inhibitor from bovine pancreas, trypsin inhibitor from soybean, Pefabloc SC (4-(2-aminoethyl) benzene sulphonyl fluoride), benzamidine, p-aminobenzamidine and chymostatin	Homo sapiens
Ni2+	-	Homo sapiens
PMSF	12% inhibition at 0.05 mM	Homo sapiens
Zn2+	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0031	-	Abz-DIYISRRLLGTFTY(NO2)A	-	Homo sapiens
0.0255	-	Ac-Arg-Arg-Leu-Leu-p-nitroanilide	-	Homo sapiens
0.1335	-	Ac-Arg-Ser-Leu-Lys-p-nitroanilide	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism
CaCl2	recombinant S1P-1 activity can be stimulated by Ca2+, but is not dependent on Ca2+. Increases activity of recombinant S1P-1 60% at 4 mM, higher concentrations of CaCl2 lowers the activity	Homo sapiens
K-phosphate buffer	-	Homo sapiens
KCl	increases activity of recombinant S1P-1 25% dependent on the substrate used	Homo sapiens
Na-phosphate buffer	-	Homo sapiens
NaCl	increases activity of recombinant S1P-1 25% at 50 mM dependent on the substrate used	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
91000	-	gel filtration	Homo sapiens
92300	-	sequence analysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
first 997 amino acids of human S1P lacking the C-terminal transmembrane region 103fold purified by immobilised metal affinity chromatography on Co2+ column	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.007	-	first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Trichoplusia ni egg, High Five cells	Homo sapiens
0.022	-	first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Sf21 cells, 103fold purified	Homo sapiens
0.2	-	first 997 amino acids of human S1P lacking the C-terminal transmembrane region prepared from Sf21 cells	Homo sapiens

Storage Stability

Storage Stability	Organism
-80°C, 40% (v/v) glycerol	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Abz-DIYISRRLLGTFTY(NO2)A + H2O	S1P displays pronounced positive cooperativity with this substrate derived from the viral coat glycoprotein of the lassa virus	Homo sapiens	?	-	?
Ac-Arg-Arg-Leu-Leu-p-nitroanilide + H2O	-	Homo sapiens	?	-	?
Ac-Arg-Ser-Leu-Lys-p-nitroanilide + H2O	-	Homo sapiens	?	-	?
Ac-Leu-4-methyl-coumaryl-7-amide + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
MEROPS S08.8063	-	Homo sapiens
S1P	-	Homo sapiens
SKI-1	-	Homo sapiens
subtilisin/kexin isozyme 1	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.005	-	Ac-Arg-Ser-Leu-Lys-p-nitroanilide	-	Homo sapiens
0.03	-	Ac-Arg-Arg-Leu-Leu-p-nitroanilide	-	Homo sapiens
0.046	-	Abz-DIYISRRLLGTFTY(NO2)A	-	Homo sapiens