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Literature summary for 3.4.21.112 extracted from

  • Cheng, D.; Espenshade, P.J.; Slaughter, C.A.; Jaen, J.C.; Brown, M.S.; Goldstein, J.L.
    Secreted site-1 protease cleaves peptides corresponding to luminal loop of sterol regulatory element-binding proteins (1999), J. Biol. Chem., 274, 22805-22812.
    View publication on PubMed
Search for more literature for 3.4.21.112

Cloned(Commentary)

Cloned (Comment) Organism
truncated from lacking the COOH-terminal membrane anchor, expressed in CHO cells Mammalia

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline partial inhibition at very high concentrations Mammalia
Ca2+ inhibition above 3 mM Mammalia
dithiothreitol partial inhibition at very high concentrations Mammalia
EDTA partial inhibition at very high concentrations Mammalia
EGTA partial inhibition at very high concentrations Mammalia
Pefabloc SC at high concentrations Mammalia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Ac-VFRSLK-4-methyl-coumaryl-7-amide pH 8, 37°C Mammalia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mammalia functions to control lipid biosynthesis and uptake in animal cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mammalia
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification enzyme undergoes autocatalytic processing, generating an active form S1P-B, which is further cleaved after residue 186 to generate S1P-C Mammalia

Purification (Commentary)

Purification (Comment) Organism
-
 Mammalia

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-VFRSLK-4-methyl-coumaryl-7-amide + H2O
-
 Mammalia ?
-
 ?
Ac-VFRSLK-4-methylcoumarin 7-amide
-
 Mammalia ?
-
 ?
additional information cleaves after lysine or leucine rather than arginine Mammalia ?
-
 ?
additional information functions to control lipid biosynthesis and uptake in animal cells Mammalia ?
-
 ?
sterol regulatory element-binding protein + H2O SREBP Mammalia ?
-
 ?

Synonyms

Synonyms Comment Organism
S1P
-
 Mammalia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Mammalia