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Literature summary for 3.4.21.111 extracted from
- Construction of an expression system for aqualysin I in Escherichia coli that gives a markedly improved yield of the enzyme protein (2008), Biosci. Biotechnol. Biochem., 72, 2012-2018.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | a maltose biding protein (MBP)-fused proaqualysin I expression plasmid is developed in which MBP is attached to the N-terminus of proaqualysin I. MBP appears effectively to suppress the folding-promoting activity of the N-terminal propeptide when the bacteria are grown at 30°C, leading to a massive accumulation of fusion aqualysin I precursor. The precursor is converted efficiently to mature aqualysin I by heat treatment at 70°C. By analyzing the product it is confirmed that aqualysin I is initially expressed as a whole fusion protein and then processed autocatalytically | Thermus aquaticus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a maltose biding protein-proaqualysin I fusion protein | Thermus aquaticus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S22A | inactive mutant | Thermus aquaticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
SDS-PAGE, mature aqualysin I | Thermus aquaticus |
| 80000 | - |
SDS-PAGE, maltose binding protein-proaqualysin I fusion protein | Thermus aquaticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus aquaticus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | - |
Thermus aquaticus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using hydrophobic chromatography ad cation-exchange-chromatography | Thermus aquaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O | - |
Thermus aquaticus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aqualysin I | - |
Thermus aquaticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Thermus aquaticus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermus aquaticus |
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Release 2023.1 (January 2023)
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