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Literature summary for 3.4.21.111 extracted from

  • Sakaguchi, M.; Matsuzaki, M.; Niimiya, K.; Seino, J.; Sugahara, Y.; Kawakita, M.
    Role of proline residues in conferring thermostability on aqualysin I (2007), J. Biochem., 141, 213-220.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant proteins expressed in Escherichia coli TG1 Thermus aquaticus

Protein Variants

Protein Variants Comment Organism
P240N no effect on the specific activity, almost no effect on the thermostability of the protein Thermus aquaticus
P268T no effect on the specific activity, mutation reduces the thermostability of the protein Thermus aquaticus
P5N no effect on the specific activity, mutation reduces the thermostability of the protein Thermus aquaticus
P7I no effect on the specific activity mutation strongly reduces the thermostability of the protein Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.69
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus
0.72
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P268T mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
0.74
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P7I mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
0.75
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P5N mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
0.79
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P240N mutant enzyme, 40°C, pH 7.5 Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-
Thermus aquaticus YT-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant proteins Thermus aquaticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Thermus aquaticus ?
-
 ?
casein + H2O
-
 Thermus aquaticus YT-1 ?
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?

Synonyms

Synonyms Comment Organism
aqualysin I
-
 Thermus aquaticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 P5N and P7I mutant, decrease of activity at higher temperatures Thermus aquaticus
80 100 wild type enzyme fully active Thermus aquaticus
100
-
 P240N and P268T mutant Thermus aquaticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 100 activity curves given for wild type and all mutant enzymes, no differences in activity from 40-60°C Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 P7I mutant is less thermostable than the wild type enzyme and other mutants in the study, complete loss of activity upon 2 h incubation at 70°C, half life: 60 min Thermus aquaticus
70
-
 wild type and P240N mutant retain 60% of activity after 2 h incubation at 60°C Thermus aquaticus
70 80 P268T mutant almost as stable as the wild type enzyme Thermus aquaticus
75.7
-
 transition temperature of the P7I mutant enzyme Thermus aquaticus
80
-
 P5N mutant much more thermolabile than the wild type enzyme, half life at 80°C: 25 min Thermus aquaticus
80
-
 P7I mutant is less thermostable than the wild type enzyme and other mutants in the study, half life: 10 min Thermus aquaticus
83.5
-
 transition temperature of the P5N mutant enzyme Thermus aquaticus
86.5
-
 transition temperature of the P268T mutant enzyme Thermus aquaticus
90
-
 half life of P268T mutant: 30 min Thermus aquaticus
90
-
 P5N mutant much more thermolabile than the wild type enzyme, half life at 80°C: 15 min Thermus aquaticus
90
-
 wild type and P240N mutant retain 50% of activity after 45 min incubation at 90°C Thermus aquaticus
91.8
-
 transition temperature of the P240N mutant enzyme Thermus aquaticus
94
-
 transition temperature of the wild type enzyme Thermus aquaticus
100
-
 half of the activity of the P268T mutant is lost within a few min Thermus aquaticus
100
-
 wild type and P240N mutant retain 50% of activity after 5 min incubation at 100°C Thermus aquaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.24
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus
2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P5N mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
66.5
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P7I mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
72.1
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P5N mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
85.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P240N mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
88.7
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide P268T mutant enzyme, 40°C, pH 7.5 Thermus aquaticus
98.1
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide wild type enzyme, 40°C, pH 7.5 Thermus aquaticus