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Literature summary for 3.4.21.111 extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease (1999), J. Biochem., 125, 1016-1021.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus aquaticus

Protein Variants

Protein Variants Comment Organism
G131D reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
G131H reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
G131K reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
S102E reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
S102H reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus
S102K reduced efficiency for N-succinyl-Phe-Ala-Ala-4-nitroanilide, raised kcat for N-succinyl-Ala-Ala-Ala-4-nitroanilide Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.044
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
0.15
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
0.24
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
0.34
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
0.49
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
0.51
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
0.52
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
1.1
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1.7
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
1.8
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
1.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
2.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
2.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
2.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
3.8
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
7.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
10
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
0.11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
0.5
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
1.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
1.2
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
1.5
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
1.6
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
2.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
2.9
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
5.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
7.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
7.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131D mutant Thermus aquaticus
8.5
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus
8.9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131H mutant Thermus aquaticus
9
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, G131K mutant Thermus aquaticus
11
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
25
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202E mutant Thermus aquaticus
33
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, wild-type Thermus aquaticus
54
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202H mutant Thermus aquaticus
144
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.5, 40°C, S202K mutant Thermus aquaticus