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Literature summary for 3.4.21.111 extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Substrate specificity of aqualysin I, a bacterial thermophilic alkaline serine protease from Thermus aquaticus YT-1: comparison with proteinase K, subtilisin BPN' and subtilisin Carlsberg (1998), Biosci. Biotechnol. Biochem., 62, 2161-2165.
    View publication on PubMed
Search for more literature for 3.4.21.111

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.039
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.04
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.064
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.087
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.41
-
 N-succinyl-Phe-Val-Leu-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.79
-
 N-succinyl-Ala-Pro-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.91
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
1.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
1.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
1.6
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
1.6
-
 N-succinyl-Ala-Ala-Val-4-nitroanilide pH 7.4, 40°C Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
 YT-1
-
Thermus aquaticus YT-1
-
 YT-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme has 3 subsites S1, S2 and S3 in its substrate binding site. S1 site prefers alanine and phenylalanine, S2 site prefers alanine and norleucine, S3 site prefers phenylalanine and isoleucine Thermus aquaticus ?
-
 ?
additional information enzyme has 3 subsites S1, S2 and S3 in its substrate binding site. S1 site prefers alanine and phenylalanine, S2 site prefers alanine and norleucine, S3 site prefers phenylalanine and isoleucine Thermus aquaticus YT-1 ?
-
 ?
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Val-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Val + 4-nitroaniline
-
 ?
N-succinyl-Ala-Pro-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Pro-Ala + 4-nitroaniline
-
 ?
N-succinyl-Gly-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Gly-betaAla-Ala + 4-nitroaniline
-
 ?
N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Gly-Gly-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ile-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-betaAla-Ala + 4-nitroaniline
-
 ?
N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
N-succinyl-Leu-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Leu-betaAla-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-betaAla-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Leu-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Leu-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Ala + 4-nitroaniline
-
 ?
N-succinyl-Phe-Val-Leu-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Leu + 4-nitroaniline
-
 ?
N-succinyl-Phe-Val-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Phe + 4-nitroaniline
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
 N-succinyl-Ala-Ala-Val-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.069
-
 N-succinyl-Phe-Val-Leu-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.15
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
0.48
-
 N-succinyl-Ala-Pro-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
1.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
2.5
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.3
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.6
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
5
-
 N-succinyl-Phe-Val-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
8.8
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus