Literature summary for 3.4.21.111 extracted from

Tanaka, T.; Matsuzawa, H.; Kojima, S.; Kumagai, I.; Miura, K.; Ohta, T.
P1 specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor (1998), Biosci. Biotechnol. Biochem., 62, 2035-2038.

Search for more literature for 3.4.21.111

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.036	-	N-succinyl-Phe-Nle-Ala-4-nitroanilide	pH 7.5, 40°C	Thermus aquaticus
1.2	-	N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide	pH 7.5, 40°C	Thermus aquaticus

Organism

Organism	UniProt	Comment	Textmining
Thermus aquaticus	-	YT-1	-
Thermus aquaticus YT-1	-	YT-1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O	-	Thermus aquaticus	N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline	-	?
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O	-	Thermus aquaticus YT-1	N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline	-	?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O	-	Thermus aquaticus	N-succinyl-Phe-Nle-Ala + 4-nitroaniline	-	?
N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O	-	Thermus aquaticus YT-1	N-succinyl-Phe-Nle-Ala + 4-nitroaniline	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.3	-	N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide	pH 7.5, 40°C	Thermus aquaticus
8.8	-	N-succinyl-Phe-Nle-Ala-4-nitroanilide	pH 7.5, 40°C	Thermus aquaticus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)