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Literature summary for 3.4.21.111 extracted from

  • Lin, S.J.; Kim, D.W.; Ryugo, Y.; Wakagi, T.; Matsuzawa, H.
    Increase of the protease activity of aqualysin I, a thermostable serine protease, by replacing Asn219 near the catalytic residue Ser222 (1997), Biosci. Biotechnol. Biochem., 61, 718-719.
    View publication on PubMed
Search for more literature for 3.4.21.111

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus aquaticus

Protein Variants

Protein Variants Comment Organism
N219S increased catalytic activity Thermus aquaticus
N219T slightly decreased catalytic activity Thermus aquaticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
0.5
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus
0.7
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus
1.1
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
1.3
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus
2.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus
2.6
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
2.7
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus
2.9
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus

Organism

Organism UniProt Comment Textmining
Thermus aquaticus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Val-Ala + 4-nitroaniline
-
 ?
N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide + H2O
-
 Thermus aquaticus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 rapid inactivation in absence of Ca2+ Thermus aquaticus
90
-
 half-life of wild-type and N219S mutant: 120 min, half-life of N219T mutant: 60 min in presence of Ca2+ Thermus aquaticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
17
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
18
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus
24
-
 N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus
26
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus
42
-
 N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus
66
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, N219T mutant Thermus aquaticus
84
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, wild-type Thermus aquaticus
296
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide 40°C pH 7.5, N219S mutant Thermus aquaticus