Cloned (Comment) | Organism |
---|---|
amino acid residues 31-1032 of the C5a peptidase gene are fused to GST and expressed in Escherichia coli. In addition expression as an N-terminal His-tagged C5a fusion protein in Escherichia coli | Streptococcus pyogenes |
Crystallization (Comment) | Organism |
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crystal structure of an active form of ScpA is solved to 1.9 A resolution. The ScpA structure reveals that access to the catalytic site is restricted by variable regions in the catalytic domain and by the presence of the inserted protease-associated domain and the second fibronectin type III domains (Fn2). Modeling of the ScpA-C5a complex indicates that the substrate binds with carboxyl-terminal residues (65-74) extended through the active site and core residues (1-64) forming exosite-type interactions with the Fn2 domain. Substrate binding is anticipated to be dominated by ionic interactions in two distinct regions of ScpA. On the prime side of the active site, salt bridges are predicted between P1', P2', and P7' residues, and residues in the catalytic and PA domains. Remote to the active site, a larger number of ionic interactions between residues in the C5a core and the Fn2 domain are observed in the model. Thus, both PA and Fn2 domains are expected to play significant roles in substrate recognition | Streptococcus pyogenes |
Organism | UniProt | Comment | Textmining |
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Streptococcus pyogenes | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-terminal His-tagged C5a fusion protein + H2O | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis of cleaved HT-C5a indicate a loss of 830 Da, consistent with the removal of seven residues from the C-terminus | Streptococcus pyogenes | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C5a peptidase | - |
Streptococcus pyogenes |
SCPA | - |
Streptococcus pyogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Streptococcus pyogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Streptococcus pyogenes |