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Literature summary for 3.4.21.110 extracted from
- Characterization of the streptococcal C5a peptidase using a C5a-green fluorescent protein fusion protein substrate (2000), J. Bacteriol., 182, 3254-3258.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D130A | mutant with reduced proteolytic activity | Streptococcus pyogenes |
| H193A | mutant with reduced proteolytic activity | Streptococcus pyogenes |
| L512A | mutant with reduced proteolytic activity | Streptococcus pyogenes |
| N295A | mutant with reduced proteolytic activity | Streptococcus pyogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibition at 10 mM | Streptococcus pyogenes |  |
| Mn2+ | inhibition at 10 mM | Streptococcus pyogenes | |
| additional information | no inhibitor: EDTA | Streptococcus pyogenes | |
| NaCl | - |
Streptococcus pyogenes | |
| Zn2+ | significant inhibition at 0.1-10 mM | Streptococcus pyogenes | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chemotaxin C5a | Streptococcus pyogenes | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pyogenes | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptococcus pyogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chemotaxin C5a | - |
Streptococcus pyogenes | ? | - |
? | |
| chemotaxin C5a + H2O | - |
Streptococcus pyogenes | ? | - |
? | |
| glutathione S-transferase-chemotaxin C5a-GFP + H2O | fusion protein | Streptococcus pyogenes | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| S08.020 | Merops ID | Streptococcus pyogenes |
| SCPA | - |
Streptococcus pyogenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
85% activity after 30 min, 75% activity after 60 min | Streptococcus pyogenes |
| 100 | - |
30% activity after boiling for 10 min | Streptococcus pyogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptococcus pyogenes |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity sharply declines below pH 7 | Streptococcus pyogenes |
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Release 2023.1 (January 2023)
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