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Literature summary for 3.4.21.109 extracted from

  • Buzza, M.S.; Martin, E.W.; Driesbaugh, K.H.; Desilets, A.; Leduc, R.; Antalis, T.M.
    Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway (2013), J. Biol. Chem., 288, 10328-10337.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.21.109

Activating Compound

Activating Compound Comment Organism Structure
prostasin prostasin stimulates the conversion of the cell-associated 70-kDa matriptase zymogen to the two-chain active form. Prostasin induces matriptase zymogen activation in intestinal epithelial cells to regulate closure of the paracellular pathway. But active recombinant matriptase, however, does not require the expression of endogenous prostasin for barrier-forming activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information siRNA silencing of matriptase expression Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
hepatocyte growth factor activator inhibitor 1 HAI-1, a Kunitz-type inhibitor that functions both as a chaperone and a reversible inhibitor Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE Homo sapiens
70000
-
 x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
proform epithelial sodium channel + H2O Homo sapiens
-
 mature epithelial sodium channel + ?
-
 ?
proform G protein-coupled protease activated receptor-2 + H2O Homo sapiens
-
 mature G protein-coupled protease activated receptor-2 + ?
-
 ?
proform prostasin + H2O Homo sapiens
-
 mature prostasin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the matriptase zymogen performs its own activation via autoproteolysis, also mutants S805A-matriptase and G827R-matriptase are readily cleaved to the two-chain form by recombinant matriptase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
-
 Homo sapiens
-
epidermis
-
 Homo sapiens
-
epithelium
-
 Homo sapiens
-
intestine
-
 Homo sapiens
-
skin
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
proform epithelial sodium channel + H2O
-
 Homo sapiens mature epithelial sodium channel + ?
-
 ?
proform G protein-coupled protease activated receptor-2 + H2O
-
 Homo sapiens mature G protein-coupled protease activated receptor-2 + ?
-
 ?
proform prostasin + H2O
-
 Homo sapiens mature prostasin + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, matriptase zymogen, SDS-PAGE, x * 30000, matriptase serine protease domain, SDS-PAGE Homo sapiens

General Information

General Information Comment Organism
malfunction siRNA silencing of matriptase expression or inhibition of matriptase activity impairs development of transepithelial electrical resistance and increases the permeability of Caco-2 epithelial barriers Homo sapiens
metabolism the matriptase zymogen is capable of intermolecular autoproteolytic activation, and the active enzyme is also an effective activator of the prostasin zymogen through direct proteolytic cleavage at its zymogen activation site. Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway. Activated prostasin appears to target several downstream effector proteins, including the epithelial sodium channel and the G protein-coupled protease activated receptor-2, which are both matriptase substrates. Matriptase and not prostasin is the primary effector protease of tight junction assembly in simple columnar epithelia Homo sapiens
physiological function matriptase promotes intestinal epithelial barrier formation Homo sapiens