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Literature summary for 3.4.21.109 extracted from
- The activity of a type II transmembrane serine protease, matriptase, is dependent solely on the catalytic domain (2009), Biosci. Biotechnol. Biochem., 73, 454-456.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| two soluble variants, containing the entire extracellular domains, Tyr81 - Val855, and the catalytic domain, Asp603 - Val855, are produced, the vectors pT7blue2 and pSec-ek-MT-SP1 are used | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00352 | - |
single-chain urokinase-type plasminogen activator | extracellular domains, Tyr81 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
| 0.00599 | - |
single-chain urokinase-type plasminogen activator | catalytic domain, Asp603 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
| 0.582 | - |
t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-methyl-coumaryl-7-amide | catalytic domain, Asp603 - Val855, after treatment with enteropeptidase | Rattus norvegicus |  |
| 0.59 | - |
t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-methyl-coumaryl-7-amide | extracellular domains, Tyr81 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
catalytic domain, Asp603 - Val855, after treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
| 28000 | - |
extracellular domains, Tyr81 - Val855, after treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
| 30000 | - |
catalytic domain, Asp603 - Val855, before treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
| 35000 | - |
catalytic domain, Asp603 - Val855, before treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
| 40000 | - |
catalytic domain, Asp603 - Val855, before treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
| 95000 | - |
extracellular domains, Tyr81 - Val855, before treatment with enteropeptidase, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pro-hepatocyte growth factor + H2O | Rattus norvegicus | - |
? | - |
? | |
| prostasin + H2O | Rattus norvegicus | - |
? | - |
? | |
| single-chain urokinase-type plasminogen activator + H2O | Rattus norvegicus | sc-uPA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| enterocyte | - |
Rattus norvegicus | - |
| epithelial cell | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pro-hepatocyte growth factor + H2O | - |
Rattus norvegicus | ? | - |
? | |
| prostasin + H2O | - |
Rattus norvegicus | ? | - |
? | |
| single-chain urokinase-type plasminogen activator + H2O | sc-uPA | Rattus norvegicus | ? | - |
? | |
| t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-methyl-coumaryl-7-amide + H2O | activity assay | Rattus norvegicus | t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| matriptase | - |
Rattus norvegicus |
| membrane-type serine protease 1 | - |
Rattus norvegicus |
| MT-SP1 | - |
Rattus norvegicus |
| type II transmembrane serine protease | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.57 | - |
single-chain urokinase-type plasminogen activator | catalytic domain, Asp603 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
| 0.71 | - |
single-chain urokinase-type plasminogen activator | extracellular domains, Tyr81 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
| 13.1 | - |
t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-methyl-coumaryl-7-amide | extracellular domains, Tyr81 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
| 13.7 | - |
t-butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-methyl-coumaryl-7-amide | catalytic domain, Asp603 - Val855, after treatment with enteropeptidase | Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
activity assay | Rattus norvegicus |
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Release 2023.1 (January 2023)
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