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Literature summary for 3.4.21.109 extracted from

  • Chen, M.; Chen, L.M.; Lin, C.Y.; Chai, K.X.
    The epidermal growth factor receptor (EGFR) is proteolytically modified by the Matriptase-Prostasin serine protease cascade in cultured epithelial cells (2007), Biochim. Biophys. Acta, 1783, 896-903.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of prostasin and HA-tagged matripatse in FT-293 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
epidermal growth factor receptor + H2O Homo sapiens the epidermal growth factor receptor, EGFR is proteolytically cleaved in the N-terminal extracellular domain by the matriptase-prostasin serine protease cascade in cultured epithelial cells EGFR135 + EGFR110 fragments of 135 and 110 kDa, no longer responsive to EGF stimulation ?
prostasin + H2O Homo sapiens proteolytic activation by matriptase, when expressed without matriptase, prostasin remains in the zymogen form and no prostasin-PN-1 complex is formed, overview activated prostasin + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
BEAS-2B cell normal lung epithelial cells, matriptase-prostasin cascade Homo sapiens
-
BPH-1 cell benign prostate hyperplasia, matriptase-prostasin cascade Homo sapiens
-
epithelial cell matriptase-prostasin cascade Homo sapiens
-
MDA-MB-231 cell breast cancer cell line, matriptase-prostasin cascade Homo sapiens
-
additional information no endogenous expression of matriptase, prostasin or EGFR in HEK-293 cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
epidermal growth factor receptor + H2O
-
Homo sapiens EGFR135 + EGFR110 fragments of 135 and 110 kDa, EGFR110 is constitutively active, while EGFR135 is inactive in terms of tyrosine phosphorylation ?
epidermal growth factor receptor + H2O the epidermal growth factor receptor, EGFR is proteolytically cleaved in the N-terminal extracellular domain by the matriptase-prostasin serine protease cascade in cultured epithelial cells Homo sapiens EGFR135 + EGFR110 fragments of 135 and 110 kDa, no longer responsive to EGF stimulation ?
prostasin + H2O proteolytic activation by matriptase, when expressed without matriptase, prostasin remains in the zymogen form and no prostasin-PN-1 complex is formed, overview Homo sapiens activated prostasin + ?
-
?
prostasin + H2O proteolytic activation by matriptase Homo sapiens activated prostasin + ?
-
?