Cloned (Comment) | Organism |
---|---|
co-expression of prostasin and HA-tagged matripatse in FT-293 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
epidermal growth factor receptor + H2O | Homo sapiens | the epidermal growth factor receptor, EGFR is proteolytically cleaved in the N-terminal extracellular domain by the matriptase-prostasin serine protease cascade in cultured epithelial cells | EGFR135 + EGFR110 | fragments of 135 and 110 kDa, no longer responsive to EGF stimulation | ? | |
prostasin + H2O | Homo sapiens | proteolytic activation by matriptase, when expressed without matriptase, prostasin remains in the zymogen form and no prostasin-PN-1 complex is formed, overview | activated prostasin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
BEAS-2B cell | normal lung epithelial cells, matriptase-prostasin cascade | Homo sapiens | - |
BPH-1 cell | benign prostate hyperplasia, matriptase-prostasin cascade | Homo sapiens | - |
epithelial cell | matriptase-prostasin cascade | Homo sapiens | - |
MDA-MB-231 cell | breast cancer cell line, matriptase-prostasin cascade | Homo sapiens | - |
additional information | no endogenous expression of matriptase, prostasin or EGFR in HEK-293 cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epidermal growth factor receptor + H2O | - |
Homo sapiens | EGFR135 + EGFR110 | fragments of 135 and 110 kDa, EGFR110 is constitutively active, while EGFR135 is inactive in terms of tyrosine phosphorylation | ? | |
epidermal growth factor receptor + H2O | the epidermal growth factor receptor, EGFR is proteolytically cleaved in the N-terminal extracellular domain by the matriptase-prostasin serine protease cascade in cultured epithelial cells | Homo sapiens | EGFR135 + EGFR110 | fragments of 135 and 110 kDa, no longer responsive to EGF stimulation | ? | |
prostasin + H2O | proteolytic activation by matriptase, when expressed without matriptase, prostasin remains in the zymogen form and no prostasin-PN-1 complex is formed, overview | Homo sapiens | activated prostasin + ? | - |
? | |
prostasin + H2O | proteolytic activation by matriptase | Homo sapiens | activated prostasin + ? | - |
? |