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Literature summary for 3.4.21.109 extracted from
- Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) (2006), FEBS J., 273, 615-627.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | small interfering RNAs for matriptase efficiently block both the matriptase expression and the cleavage of IGBP-rP1 in OVISE cells | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | inhibition of IGFBP-rP1 processing | Homo sapiens |  |
| Aprotinin | inhibition of IGFBP-rP1 processing | Homo sapiens | |
| hepatocyte growth factor activator inhibitor-1 | HAI-1, forms complexes wih the enzyme in vivo | Homo sapiens | |
| additional information | no inhibition of IGFBP-rP1 processing by leupeptin, pepstatin, and N-(R)-(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-l-3-(2'-naphthyl)alaninyl-l-alanine 2-aminoethyl amide, i.e. TAPI-1; not inhibited by leupeptin, pepstatin and N-(R)-(2-(hydroxyaminocarbonyl)methyl)-4-methylpentanoyl-L-3-(2'-naphthyl)alaninyl-L-alanine 2-aminoethyl amide | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell surface | - |
Homo sapiens | 9986 | - |
| membrane | - |
Homo sapiens | 16020 | - |
| membrane | bound | Homo sapiens | 16020 | - |
| soluble | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gelatin zymography, soluble active enzyme | Homo sapiens |
| 80000 | - |
immunoblotting, membrane-bound enzyme | Homo sapiens |
| 95000 | - |
gelatin zymography or immunoblotting, matripase/hepatocyte growth factor activator inhibitor complex | Homo sapiens |
| 110000 | - |
gelatin zymography or immunoblotting, matripase/hepatocyte growth factor activator inhibitor complex | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | Homo sapiens | - |
? | - |
? | |
| insulin-like growth factor binding protein-related protein-1 + H2O | Homo sapiens | proteolytical cleavage on the cell surface of a a single-chain IGFB-rP1 to a two-chain form, consisting of a 25 kDa chain and an 8 kDa chain, changing its biological in modulation of cellular adhesion and growth in an IGF/insulin-dependent or independent manner, as well as tumor-suppressive activity, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by molecular-sieve chromatography, reverse-phase HPLC | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| DLD-1 cell | - |
Homo sapiens | - |
| DLD-1 cell | major amount of matripase | Homo sapiens | - |
| HEK-293 cell | - |
Homo sapiens | - |
| HSC-4 cell | - |
Homo sapiens | - |
| MKN-45 cell | major amount of matripase | Homo sapiens | - |
| MKN-45 cell | gastric carcinoma cells, high level expression of IGFBP-rP1-cleaving enzyme | Homo sapiens | - |
| additional information | no IGFBP-rP1 processing activity in HSC-4 cells, HEK-293 cells, HLE cells, MKN-45 cells, HT-1080 cells, EJ-1 cells, and ECV-304 cells, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium | Homo sapiens | - |
| ovary adenocarcinoma cell line | major amount of matripase | Homo sapiens | - |
| OVISE cell | ovarian clear cell adenocarcinoma cells, high level expression of IGFBP-rP1-cleaving enzyme | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | - |
Homo sapiens | ? | - |
? | |
| insulin growth factor binding protein-related protein-1 + H2O | cleaved by the soluble form of active matripase | Homo sapiens | ? | - |
? | |
| insulin-like growth factor binding protein-related protein-1 + H2O | proteolytical cleavage on the cell surface of a a single-chain IGFB-rP1 to a two-chain form, consisting of a 25 kDa chain and an 8 kDa chain, changing its biological in modulation of cellular adhesion and growth in an IGF/insulin-dependent or independent manner, as well as tumor-suppressive activity, the soluble form of active matriptase cleaves IGFBP-rP1 but HAI-1 or some other inhibitors block its activity in culture medium, overview | Homo sapiens | ? | - |
? | |
| insulin-like growth factor binding protein-related protein-1 + H2O | i.e. IGFBP-rP1 or angiomodulin or mac25 | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000-80000, single-chain latent enzyme, SDS-PAGE, x * 95000-110000, active enzyme in complex with inhibitor HAI-1, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| membrane-type serine proteinase matripase | - |
Homo sapiens |
| membrane-type serine proteinase matriptase | - |
Homo sapiens |
| MT-SP1 | - |
Homo sapiens |
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