Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
comparison of wild-type and mutan S276C. In wild-type, water molecule number 377 forms an interaction between side chains of S276 and I270* of the adjacent molecule. In mutant S276C, this water molecule is absent | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R280A | inactive | Homo sapiens |
R280A/S276C | inactive | Homo sapiens |
S276C | inactive. Mutation does not affect the oligomeric properties and maintains proper hydrogen bonding distances in the active-site triad residues | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43464 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-casein + H2O | - |
Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
74 | - |
melting temperature, mutant S276C | Homo sapiens |