Cloned (Comment) | Organism |
---|---|
ubiquitin carboxyl-terminal hydrolase L1 and HtrA2 are expressed as GST-fusion proteins in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
S306A | protease-inactive mutant | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin carboxyl-terminal hydrolase L1 + H2O | Mus musculus | natural substrate for HtrA2 in the apoptotic pathway. HtrA2 directly cleaves UCH-L1 and inhibits its hydrolase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione 4B bead chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MEF cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin carboxyl-terminal hydrolase L1 + H2O | natural substrate for HtrA2 in the apoptotic pathway. HtrA2 directly cleaves UCH-L1 and inhibits its hydrolase activity | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high temperature requirement A2 protease | - |
Mus musculus |
HtrA2 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | cytosolic HtrA2 decreases ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) protein level and its hydrolase activity through HtrA2-mediated cleavage of UCH-L1 under apoptotic conditions. HtrA2-mediated cleavage of UCH-L1 plays important roles in regulating the fine balance between cell growth and cell death | Mus musculus |