Literature summary for 3.4.21.108 extracted from

Kuninaka, S.; Iida, S.I.; Hara, T.; Nomura, M.; Naoe, H.; Morisaki, T.; Nitta, M.; Arima, Y.; Mimori, T.; Yonehara, S.; Saya, H.
Serine protease Omi/HtrA2 targets WARTS kinase to control cell proliferation (2007), Oncogene, 26, 2395-2406.

Search for more literature for 3.4.21.108

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	activation of Omi by UV radiation	Homo sapiens
additional information	apoptotic stimuli induce the activation of Omi and the consequent digestion of WTS by a caspase-independent mechanism	Mus musculus
staurosporine	-	Homo sapiens
staurosporine	activates protease activity of Omi leading to cell death. Induces cell death in wild-type MEFs but not in caspase-9-deficient MEFs	Mus musculus
WTS	activator of Omi protease	Mus musculus

Application

Application	Comment	Organism
additional information	a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death	Mus musculus
additional information	a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death	Homo sapiens
additional information	a role for Omi-mediated processing of WTS in negative regulation of cell cycle progression at interphase. Omi protease is not necessarily required for cell death	synthetic construct

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli (BL21) transformed with pRSET containing cDNA for full-length wild-type Omi or mutant	synthetic construct

Protein Variants

Protein Variants	Comment	Organism
S306A	inactive, has no effect on the abundance of WTS	synthetic construct
Y361A	mature Omi with PDZ domain mutation, cannot interact with C-terminus of WTS, fails to cleave full-length WTS	synthetic construct

Inhibitors

Inhibitors	Comment	Organism	Structure
N-tosyllysine chloromethyl ketone	-	Homo sapiens
N-tosyllysine chloromethyl ketone	blocks ability of wild-type Omi to proteolyse WTS	synthetic construct
siRNA	reduces endogenous Omi expression in HeLa cells	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-
nucleus	-	Homo sapiens	5634	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Mus musculus	-	-	-
synthetic construct	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
immunopurified from HEK-293 T cells	Homo sapiens
on Ni2+ agarose beads	synthetic construct

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Homo sapiens	-
HeLa cell	full length Omi present in mitochondria and nucleus	Homo sapiens	-
L-929 cell	-	Mus musculus	-
MEF cell	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
WTS + H2O	-	Homo sapiens	?	-	?
WTS + H2O	-	synthetic construct	?	-	?
WTS + H2O	is a non-apoptotic substrate of Omi	Mus musculus	?	-	?

Synonyms

Synonyms	Comment	Organism
HtrA2	-	Mus musculus
HtrA2	-	Homo sapiens
HtrA2	-	synthetic construct
Omi	-	Mus musculus
Omi	-	Homo sapiens
Omi	-	synthetic construct
Omi protease	-	Mus musculus
Omi protease	-	Homo sapiens
Omi protease	-	synthetic construct
Omi/HtrA2	-	Mus musculus
Omi/HtrA2	-	Homo sapiens
Omi/HtrA2	-	synthetic construct
serine protease Omi/HtrA2	-	Mus musculus
serine protease Omi/HtrA2	-	Homo sapiens
serine protease Omi/HtrA2	-	synthetic construct

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Release 2023.1 (January 2023)