Literature summary for 3.4.21.108 extracted from

Seong, Y.M.; Park, H.J.; Seong, G.H.; Choi, J.Y.; Yoon, S.J.; Min, B.R.; Kang, S.; Rhim, H.
N-terminal truncation circumvents proteolytic degradation of the human HtrA2/Omi serine protease in Escherichia coli: rapid purification of a proteolytically active HtrA2/Omi (2004), Protein Expr. Purif., 33, 200-208.

Search for more literature for 3.4.21.108

Cloned(Commentary)

Cloned (Comment)	Organism
barely expressed full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21	Homo sapiens
successful expression of full-length HtrA2/Omi as a GST fusion protein in Escherichia coli BL21 as compared to human HtrA2/Omi	Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	44000	low molecular masses detected due to full-length HtrA2/Omi regions or amino acid residues that are very susceptible to a high degree of proteolytic events in Escherichia coli expression systems	Homo sapiens
77000	-	-	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O43464	-	-
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
rapid purification of full-length HtrA2/Omi as a GST fusion under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads	Homo sapiens
rapid purification of GST-HtrA2/Omi fusion proteins under non-denaturing conditions by selective binding to glutathione-Sepharose 4B beads	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	from fetus	Homo sapiens	-

Storage Stability

Storage Stability	Organism
-70°C, Tris-HCl buffer, pH 7.6, 20% glycerol, several months	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-casein + H2O	-	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
HtrA2/Omi	-	Mus musculus
HtrA2/Omi serine protease	-	Homo sapiens

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Release 2023.1 (January 2023)