Application | Comment | Organism |
---|---|---|
medicine | involvement of HtrA2 in the etiology of Alzheimer's disease | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
subcloned into pET28a and propageted in Escherichia coli DH5alpha, expressed in Escherichia coli BL21 (DE3) pLys, overexpression in K269 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | mainly localized | Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme by ProBondTM resin | Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, phosphate buffered saline, pH 7.4, 20% glycerol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
A beta 40 | HtrA2/Omi binds preferentially to the short form of A beta peptides, the enzyme does not perform degradation by direct hydrolysis | Homo sapiens | ? | - |
? | |
A beta 42 | HtrA2/Omi binds poorly to A beta 42, the enzyme does not perform degradation by direct hydrolysis | Homo sapiens | ? | - |
? | |
alpha-secretase peptide | HtrA2/Omi does not perform degradation by direct hydrolysis | Homo sapiens | ? | - |
? | |
beta-secretase peptide | HtrA2/Omi does not perform degradation by direct hydrolysis | Homo sapiens | ? | - |
? | |
casein + H2O | strong hydrolytic activity | Homo sapiens | ? | - |
? | |
gamma-secretase peptide | HtrA2/Omi binds less strongly to gamma-secretase substrate peptide as compared to alpha-secretase substrate peptide and beta-secretase substrate peptide, the enzyme does not perform degradation by direct hydrolysis | Homo sapiens | ? | - |
? | |
additional information | does not bind the reversed control peptide A beta 41-1 and peptide H2-Opt | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HtrA2/Omi | - |
Homo sapiens |